Detection and Analysis of Low-Abundance Cell-Surface Biomarkers Using Enzymatic Amplification in Microfluidic Droplets

Finding the few: Cell-surface proteins are useful disease biomarkers, but current high-throughput methods are limited to detecting cells expressing more than several hundred proteins. Enzymatic amplification in microfluidic droplets (see picture) is a high-throughput method for detection and analysi...

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Bibliographic Details
Published in:	Angewandte Chemie (International ed.) Vol. 48; no. 14; pp. 2518 - 2521
Main Authors:	Joensson, Haakan N, Samuels, Michael L, Brouzes, Eric R, Medkova, Martina, Uhlén, Mathias, Link, Darren R, Andersson-Svahn, Helene
Format:	Journal Article
Language:	English
Published:	Weinheim Wiley-VCH Verlag 01-01-2009 WILEY‐VCH Verlag
Subjects:	Antigens, CD19 - analysis Biomarkers - analysis Cell Line devices droplets encapsulation enzymatic amplification Fluorescence gene high-throughput screening Humans Membrane Proteins - analysis microdroplets Microfluidic Analytical Techniques - methods microfluidics molecules picoliter droplets polymerase-chain-reaction protein expression proteins Receptors, CCR5 - analysis single cells therapy U937 Cells
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Summary:	Finding the few: Cell-surface proteins are useful disease biomarkers, but current high-throughput methods are limited to detecting cells expressing more than several hundred proteins. Enzymatic amplification in microfluidic droplets (see picture) is a high-throughput method for detection and analysis of cell-surface biomarkers expressed at very low levels on individual human cells. Droplet optical labels allow concurrent analysis of several samples.
Bibliography:	http://dx.doi.org/10.1002/anie.200804326 This work was supported in part by NIH grant R43HG003925‐01, The Knut and Alice Wallenberg Foundation, The Royal Swedish Academy of Sciences and Swedish Research Council. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1433-7851 1521-3773 1521-3773
DOI:	10.1002/anie.200804326