Serum soluble interleukin 2 receptor (sIL-2R) as a marker of acute rejection in renal transplant children

• Published in: Pediatric transplantation Vol. 16; no. 3; pp. 274 - 279
• Main Authors: García-Roca, P., Vargas, Y. A., Fuentes, Y., Hernández, A. M., Ortiz, L., Valverde, S., Velásquez-Jones, L., Romero-Navarro, B., Ramón-Garcia, G., Medeiros, M.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01-05-2012; Blackwell
• Subjects: acute cellular rejection; Adolescent; Biological and medical sciences; biomarkers; Biopsy; Child; Children; Enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; Female; Gene Expression Regulation; General aspects; Glomerular Filtration Rate; Graft Rejection; Graft Survival; Humans; interleukin 2; Interleukin 2 receptors; Kidney transplantation; Kidney Transplantation - adverse effects; Kidney Transplantation - methods; Male; Medical sciences; Pediatrics; Prospective Studies; Receptors, Interleukin-2 - blood; Reproducibility of Results; ROC Curve; Serum levels; soluble interleukin 2 receptor; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Human; Graft(material); Pediatrics; Acute; biomarkers; Cytokine; Biological marker; Transplantation; Soluble form; Homotransplantation; acute cellular rejection; Kidney; Rejection; Treatment; Urinary system; Interleukin 2; Interleukin 2 receptor; Surgery; Graft; Serum; Cell; Child; soluble interleukin 2 receptor
• ISSN: 1397-3142; 1399-3046
• DOI: 10.1111/j.1399-3046.2012.01645.x

García‐Roca P, Vargas YA, Fuentes Y, Hernández AM, Ortiz L, Valverde S, Velásquez‐Jones L, Romero‐Navarro B, Ramón‐Garcia G, Medeiros M. Serum soluble interleukin 2 receptor (sIL‐2R) as a marker of acute rejection in renal transplant children.
:  The aim of the study was to evaluate whether or not serum levels of soluble interleukin 2 receptor (sIL‐2R) predict acute rejection in pediatric recipients. We studied 51 pediatric renal transplant recipients divided into three groups: Group 1) Biopsy‐proven cellular acute rejection (n = 19), Group 2) Graft dysfunction with histological diagnosis other than acute rejection (n = 8) and Group 3) Patients with stable graft function, no biopsy (n = 24). Serum samples for sIL‐2R measurement by sandwich ELISA were obtained at the time of renal transplant and at the time of renal biopsy due to graft dysfunction (Groups 1 and 2) or at six months post‐transplant in the case of Group 3. The mean ± s.e. serum values of sIL‐2R were higher in patients during acute rejection (6539 ± 1802 pg/mL) compared to patients with other causes of graft dysfunction (2217 ± 256 pg/mL) or stable graft function at six months (2183 ± 283 pg/mL) (Kruskal–Wallis p = 0.004). When the sIL2‐R levels at the time of transplant were compared to those at the time of biopsy (Groups 1 and 2) or at six months post‐transplant in Group 3, there was no significant difference between baseline and biopsy in the acute rejection group (paired t‐test = 0.07), whereas there was a significant reduction in Groups 2 and 3.