Free radical scavenging activity of Lactobacillus fermentum in vitro and its antioxidative effect on growing-finishing pigs

• Published in: Journal of applied microbiology Vol. 107; no. 4; pp. 1140 - 1148
• Main Authors: Wang, A.N, Yi, X.W, Yu, H.F, Dong, B, Qiao, S.Y
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01-10-2009; Blackwell Publishing Ltd; Blackwell
• Subjects: Animals; Antioxidants - metabolism; Antioxidants - pharmacology; antioxidative capacity; Biological and medical sciences; Biphenyl Compounds - metabolism; Catalase - metabolism; Dietary Supplements; free radical; Free Radical Scavengers; Fundamental and applied biological sciences. Psychology; Glutathione Peroxidase - metabolism; Hydrogen Peroxide - pharmacology; Hydroxyl Radical - metabolism; Lactobacillus; Lactobacillus fermentum; Lactobacillus fermentum - growth & development; Lactobacillus fermentum - physiology; Liver - enzymology; Liver - metabolism; Malondialdehyde - metabolism; Microbiology; Muscles - enzymology; Muscles - metabolism; Picrates - metabolism; Superoxide Dismutase - metabolism; Superoxides - metabolism; Swine; Lactic acid bacteria; Lactobacillus fermentum; Applied microbiology; antioxidative capacity; Antioxidant; In vitro; Free radical; Pig; Vertebrata; Mammalia; Swine; Bacteria; Lactobacillaceae; Artiodactyla; Ungulata
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/j.1365-2672.2009.04294.x

To evaluate the free radical-scavenging capacity of Lactobacillus fermentum and its effects on antioxidant enzyme levels in finishing pigs. The free radical-scavenging activity of Lact. fermentum was analysed in vitro. The tested Lactobacillus showed a high scavenging ability against DPPH (1,1-diphenyl-2-picrylhydrazyl), superoxide and hydroxyl radicals which was dose dependent. Subsequently, 108 crossbred pigs weighing 20·67 BW, were allotted to dietary treatments including a basal diet or the basal diet supplemented with either aureomycin or 10·2 x 10⁷ Lact. fermentum CFU g⁻¹ diet. Supplementation of Lact. fermentum increased total antioxidant capacity (P < 0·01) in serum from 50 kg pigs, while serum superoxide dismutase (P = 0·01) and glutathione peroxidase (P < 0·01) increased, and malondialdehyde levels decreased (P < 0·01) in 90 kg pigs. Hepatic catalase (P = 0·04), muscle superoxide dismutase (P < 0·01) and copper-zinc-superoxide dismutase were enhanced (P = 0·01), whereas malondialdehyde levels were reduced (P = 0·05) by Lact. fermentum. The free radical-scavenging capacity of Lact. fermentum was dose dependent and its supplementation improved the antioxidant status of pigs. Lactobacillus fermentum could be used to alleviate oxidative stress and increase pig performance and improve pork quality.