Genome Organizations and Functional Analyses of a Novel Gammapartitivirus from Rhizoctonia solani AG-1 IA Strain D122

• Published in: Viruses Vol. 13; no. 11; p. 2254
• Main Authors: Zhang, Meiling, He, Zhenrui, Huang, Xiaotong, Shu, Canwei, Zhou, Erxun
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 10-11-2021; MDPI
• Subjects: Amino Acid Sequence; Base Sequence; biocontrol; Biological Control Agents; Cloning; Double Stranded RNA Viruses - classification; Double Stranded RNA Viruses - genetics; Double Stranded RNA Viruses - physiology; Double Stranded RNA Viruses - ultrastructure; Double-stranded RNA; Fungal Proteins - genetics; Fungal Viruses - classification; Fungal Viruses - genetics; Fungal Viruses - physiology; Fungal Viruses - ultrastructure; Gammapartitivirus; Genome, Viral; Genomes; Growth rate; Hybridization; hypovirulence; Isometric; Laboratories; Microscopy; Mycelia; mycovirus; Open reading frames; Oryza - microbiology; Pathogenicity; Phylogenetics; Phylogeny; Plant Diseases - microbiology; Plant Diseases - prevention & control; Rhizoctonia - pathogenicity; Rhizoctonia - virology; Rhizoctonia solani; RNA polymerase; RNA, Viral - genetics; Sclerotia; Sheath blight; Transcriptome; Transcriptomes; Virion - ultrastructure; Virulence; Viruses; United States > US; China; mycovirus; Rhizoctonia solani; hypovirulence; Gammapartitivirus; biocontrol; transcriptome
• ISSN: 1999-4915; 1999-4915
• DOI: 10.3390/v13112254

Here, we describe a novel double-stranded (ds) RNA mycovirus designated Rhizoctonia solani dsRNA virus 5 (RsRV5) from strain D122 of
AG-1 IA, the causal agent of rice sheath blight. The RsRV5 genome consists of two segments of dsRNA (dsRNA-1, 1894 bp and dsRNA-2, 1755 bp), each possessing a single open reading frame (ORF). Sequence alignments and phylogenetic analyses showed that RsRV5 is a new member of the genus
in the family
. Transmission electron microscope (TEM) images revealed that RsRV5 has isometric viral particles with a diameter of approximately 20 nm. The mycovirus RsRV5 was successfully removed from strain D122 by using the protoplast regeneration technique, thus resulting in derivative isogenic RsRV5-cured strain D122-P being obtained. RsRV5-cured strain D122-P possessed the traits of accelerated mycelial growth rate, increased sclerotia production and enhanced pathogenicity to rice leaves compared with wild type RsRV5-infection strain D122. Transcriptome analysis showed that three genes were differentially expressed between two isogenic strains, D122 and D122-P. These findings provided new insights into the molecular mechanism of the interaction between RsRV5 and its host, D122 of
AG-1 IA.