Imaging the uptake of deuterated methionine in Drosophila with stimulated Raman scattering

Imaging the uptake of deuterated methionine in Drosophila with stimulated Raman scattering

Visualizing small individual biomolecules at subcellular resolution in live cells and tissues can provide valuable insights into metabolic activity in heterogeneous cells, but is challenging. Here, we used stimulated Raman scattering (SRS) microscopy to image deuterated methionine (d-Met) incorporat...

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Bibliographic Details
Published in:Frontiers in chemistry Vol. 11; p. 1141920
Main Authors: Spratt, Spencer J, Mizuguchi, Takaha, Akaboshi, Hikaru, Kosakamoto, Hina, Okada, Rina, Obata, Fumiaki, Ozeki, Yasuyuki
Format: Journal Article
Language:English
Published: Switzerland Frontiers Media S.A 29-03-2023
Subjects:
cell heterogeneity
Chemistry
deuterium
Drosophila
metabolic tracing
methionine
stimulated Raman scattering
stimulated Raman scattering
cell heterogeneity
deuterium
methionine
Drosophila
mTOR
metabolic tracing
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Summary:Visualizing small individual biomolecules at subcellular resolution in live cells and tissues can provide valuable insights into metabolic activity in heterogeneous cells, but is challenging. Here, we used stimulated Raman scattering (SRS) microscopy to image deuterated methionine (d-Met) incorporated into tissues . : Our results demonstrate that SRS can detect a range of previously uncharacterized cell-to-cell differences in d-Met distribution within a tissue at the subcellular level. : These results demonstrate the potential of SRS microscopy for metabolic imaging of less abundant but important amino acids such as methionine in tissue.
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Reviewed by: Isaac Pence, University of Texas Southwestern Medical Center, United States
Samir F. El-Mashtoly, Egypt-Japan University of Science and Technology, Egypt
Edited by: Chi Zhang, Purdue University, United States
This article was submitted to Chemical Biology, a section of the journal Frontiers in Chemistry
ISSN:2296-2646
2296-2646
DOI:10.3389/fchem.2023.1141920