Requirement of Cellular Protein CCT7 for the Replication of Fowl Adenovirus Serotype 4 (FAdV-4) in Leghorn Male Hepatocellular Cells Via Interaction with the Viral Hexon Protein

• Published in: Viruses Vol. 11; no. 2; p. 107
• Main Authors: Gao, Junfeng, Zhao, Mingliang, Duan, Xueyan, Wang, Yongqiang, Cao, Hong, Li, Xiaoqi, Zheng, Shijun J
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 27-01-2019; MDPI
• Subjects: Adenoviridae - genetics; Adenoviridae - physiology; Adenoviridae Infections - veterinary; Adenoviruses; Animals; Capsid protein; Capsid Proteins - genetics; Capsid Proteins - metabolism; CCT7; Chaperonin Containing TCP-1 - genetics; Chaperonin Containing TCP-1 - metabolism; Chickens; Chromatography; Cloning; Ectopic expression; FAdV-4; Gene Knockdown Techniques; Genomes; Hepatitis; Hexon; Host Microbial Interactions; Infections; Liver - cytology; Liver - virology; Male; Microscopy; Molecular modelling; Monoclonal antibodies; Phylogeny; Polyclonal antibodies; Poultry; Poultry Diseases - virology; Proteins; Replication; RNA Interference; RNA polymerase; RNA-mediated interference; Serogroup; Viral infections; Virus Replication; Beijing China; United States > US; China; Japan; FAdV-4; Replication; CCT7; Hexon
• ISSN: 1999-4915; 1999-4915
• DOI: 10.3390/v11020107

Fowl adenovirus serotype 4 (FAdV-4) causes hepatitis-hydropericardium syndrome (HHS), leading to severe economic losses in the poultry industry. Although the pathogenesis of FAdV-4 infection has caused much attention, the underlying molecular mechanisms remain poorly understood. Here, we identified chaperonin containing TCP-1 subunit eta (CCT7) as an interacting partner of the FAdV-4 capsid protein hexon. We found that ectopic expression of CCT7 in leghorn male hepatocellular (LMH) cells enhanced hexon expression in pRK5-flag-hexon transfected cells. On the contrary, knockdown of cellular CCT7 by RNAi markedly reduced hexon expression in FAdV-4-infected cells and suppressed viral replication. These data suggest that CCT7 is required for FAdV-4 replication and may serve as a potential target for controlling FAdV-4 infection.