Leptospira interrogans Outer Membrane Protein-Based Nanohybrid Sensor for the Diagnosis of Leptospirosis

• Published in: Sensors (Basel, Switzerland) Vol. 21; no. 7; p. 2552
• Main Authors: Verma, Vivek, Kala, Deepak, Gupta, Shagun, Kumar, Harsh, Kaushal, Ankur, Kuča, Kamil, Cruz-Martins, Natália, Kumar, Dinesh
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 06-04-2021; MDPI
• Subjects: AuN/CNF; Biosensors; Carbon fibers; Communication; Composite materials; Deoxyribonucleic acid; Diagnosis; Disease; DNA; DNA sensor; Electrochemical analysis; Electrodes; Genes; Genomics; Gold; Humans; Hybridization; Infections; Laboratories; Leptospira interrogans; Leptospira interrogans - genetics; Leptospirosis; Leptospirosis - diagnosis; Loa22 gene; Membrane Proteins; Metal Nanoparticles; Nanofibers; Sensitivity analysis; Sensors; Spectrum analysis; Storage stability; Virulence; Voltammetry; Spain; India; Leptospira interrogans; AuN/CNF; DNA sensor; Loa22 gene
• ISSN: 1424-8220; 1424-8220
• DOI: 10.3390/s21072552

Leptospirosis is an underestimated tropical disease caused by the pathogenic
species and responsible for several serious health problems. Here, we aimed to develop an ultrasensitive DNA biosensor for the rapid and on-site detection of the
gene of
using a gold nanoparticle-carbon nanofiber composite (AuN/CNF)-based screen-printed electrode. Cyclic voltammetry and electrochemical impedance were performed for electrochemical analysis. The sensitivity of the sensor was 5431.74 μA/cm
/ng with a LOD (detection limit) of 0.0077 ng/μL using cyclic voltammetry. The developed DNA biosensor was found highly specific to the
gene of
, with a storage stability at 4 °C for 180 days and a 6% loss of the initial response. This DNA-based sensor only takes 30 min for rapid detection of the pathogen, with a higher specificity and sensitivity. The promising results obtained suggest the application of the developed sensor as a point of care device for the diagnosis of leptospirosis.