Diagnostic techniques to detect cryptic leishmaniasis in dogs

This study of several techniques for detecting cryptic leishmaniasis in dogs from areas in Spain where Leishmania infantum is highly endemic concludes that immunological techniques (enzyme-linked immunosorbent assay, immunofluorescence antibody test, Western blotting, delayed-type hypersensitivity r...

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Bibliographic Details
Published in:	Clinical and diagnostic laboratory immunology Vol. 9; no. 5; pp. 1137 - 1141
Main Authors:	Iniesta, Laura, Fernández-Barredo, Salceda, Bulle, Béatrice, Gómez, M Teresa, Piarroux, Renaud, Gállego, Montserrat, Alunda, José M, Portús, Montserrat
Format:	Journal Article
Language:	English
Published:	United States American Society for Microbiology 01-09-2002
Subjects:	Animals Blotting, Western Carrier State Dog Diseases - diagnosis Dog Diseases - immunology Dog Diseases - parasitology Dogs Enzyme-Linked Immunosorbent Assay Fluorescent Antibody Technique Hypersensitivity, Delayed - diagnosis Hypersensitivity, Delayed - parasitology Leishmania infantum - isolation & purification Leishmaniasis - diagnosis Leishmaniasis - immunology Veterinary Immunology
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Summary:	This study of several techniques for detecting cryptic leishmaniasis in dogs from areas in Spain where Leishmania infantum is highly endemic concludes that immunological techniques (enzyme-linked immunosorbent assay, immunofluorescence antibody test, Western blotting, delayed-type hypersensitivity reaction, and in vitro lymphocyte proliferation assay) do not clearly differentiate between noninfected and infected asymptomatic dogs and that culture and PCR are more reliable diagnostic tools.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 Corresponding author. Mailing address: Laboratori de Parasitologia, Facultat de Farmàcia, Universitat de Barcelona, Avda. Diagonal s.n., E-08028 Barcelona, Spain. Phone: 34 93 402 45 00. Fax: 34 93 402 45 04. E-mail: mportus@farmacia.far.ub.es.
ISSN:	1071-412X 1098-6588
DOI:	10.1128/CDLI.9.5.1137-1141.2002