Removal of Aroclor 1254 by the white rot fungus Coriolus versicolor in the presence of different concentrations of Mn(IV)oxide

• Published in: International biodeterioration & biodegradation Vol. 44; no. 4; pp. 243 - 253
• Main Authors: Cloete, T.E, Celliers, L
• Format: Journal Article
• Language: English
• Published: Barking Elsevier Ltd 01-01-1999; Elsevier
• Subjects: Aroclor 1254; Biodegradation; Biodegradation of pollutants; Biological and medical sciences; Biotechnology; Catalyst activity; Coriolus versicolor; Environment and pollution; enzyme activity; Enzyme immobilization; Fundamental and applied biological sciences. Psychology; Fungi; hydrogen peroxide; Industrial applications and implications. Economical aspects; lignin; lignin peroxidase; Manganese compounds; manganese dioxide; manganese oxide; peroxidase; polychlorinated biphenyls; Biodegradation; Enzyme; Manganese peroxidase; Coriolus versicolor; Basidiomycetes; Polychlorobiphenyl; Fungi; Pollutant; Peroxidases; Manganese IV Oxides; Chlorine Organic compounds; Concentration effect; Oxidoreductases; Thallophyta
• ISSN: 0964-8305; 1879-0208
• DOI: 10.1016/S0964-8305(99)00085-2

Lignin peroxidase (LiP) plays an active role in the biodegradation of lignin and phenolic structures resembling lignin. The role of other enzymes in the biodegradation of recalcitrant compounds, e.g. manganese(II)-peroxidase, is uncertain. Solid manganese(IV)oxide addition improved the production of manganese(II)-dependant peroxidase (MnP) and H
2O
2 and increased the rate of biodegradation of Aroclor 1254 in a nitrogen-limited medium by the white rot fungus
Coriolus versicolor. MnP activity was detected 48 h after the addition of MnO
2 to the cultures and was absent in cultures that did not receive MnO
2. The rate of Aroclor 1254 removal by
C. versicolor was influenced by the concentration of MnO
2. 34.5 mM concentrations only increased the H
2O
2 production. Removal of Aroclor 1254 in the absence of MnO
2 still took place which implied the presence of (LiP) or nonspecific absorption. The cultures containing 57.5 mM MnO
2 removed ca. 84% of the initial 750 mg l
−1 Aroclor in 6 days of incubation. Cultures with no MnO
2 and 34.5 mM removed 79 and 76%, respectively. Cultures with MnP or LiP as the dominant enzyme species removed penta- and hexachlorobiphenyls at a slower rate than tri- and tetrachlorobiphenyl.