Neuroectodermal tumor of bone. Evidence for neural differentiation in a cultured cell line

• Published in: Cancer Vol. 65; no. 8; p. 1771
• Main Authors: Isayama, T, Iwasaki, H, Kikuchi, M, Yoh, S, Takagishi, N
• Format: Journal Article
• Language: English
• Published: United States 15-04-1990
• Subjects: Adolescent; Bone Neoplasms - genetics; Bone Neoplasms - pathology; Cell Differentiation; Choline O-Acetyltransferase - analysis; Chromosomes - analysis; Culture Media; Glial Fibrillary Acidic Protein - analysis; Humans; Male; Microscopy, Electron; Neoplasms, Germ Cell and Embryonal - genetics; Neoplasms, Germ Cell and Embryonal - pathology; Neurons - pathology; Oncogene Proteins - analysis; Oncogenes; Phosphopyruvate Hydratase - analysis; Rosette Formation; S100 Proteins - analysis; Tumor Cells, Cultured
• ISSN: 0008-543X
• DOI: 10.1002/1097-0142(19900415)65:8<1771::AID-CNCR2820650818>3.0.CO;2-P

A new cell line was established from a "neuroectodermal tumor of bone" affecting the right scapula of an 18-year-old man. The original neoplasm had dense proliferation of small round cells with abundant glycogen content and numerous Homer-Wright rosettes. The culture showed proliferation of small spindle cells with uniform oval nuclei and slender cytoplasmic processes. When the culture reached maximum density, rosette-like structures similar to those in the original tumor were formed. Under the influence of N6,O2'-dibutyryl adenosine 3',5'-cyclic monophosphoric acid (dibutyryl cAMP), the cultured cells expressed these rosette-like structures even in the lower cell concentration. Electron microscopy revealed that the cultured cells treated with dibutyryl cAMP contained high-density granules, well-developed microtubules, and abundant 10-nm filaments. By immunocytochemistry, neuron-specific enolase (NSE), and N-myc oncogene product were detected in the cultured cells as well as the original tumor. These results indicated the neuroectodermal origin of some of the small round cell tumors of bone.