Enhanced extraction yields and mobile phase separations by solvent mixtures for the analysis of metabolites in Annona muricata L. leaves

• Published in: Journal of separation science Vol. 32; no. 23-24; pp. 4176 - 4185
• Main Authors: Ribeiro de Souza, Eloana Benassi, da Silva, Renata Reis, Afonso, Sabrina, Scarminio, Ieda Spacino
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley-VCH Verlag 01-12-2009; WILEY-VCH Verlag; WILEY‐VCH Verlag; Wiley
• Subjects: Annona - chemistry; Annona - metabolism; Annona muricata L. leaves; Biological and medical sciences; Chemical constitution; Chromatography, High Pressure Liquid - methods; Ethyl alcohol; Extraction; Extraction yields; Fundamental and applied biological sciences. Psychology; Leaves; Metabolites; Methyl alcohol; Mobile phase strength; Organic Chemicals - isolation & purification; Organic Chemicals - metabolism; Plant Extracts - analysis; Plant Leaves - chemistry; Plant Leaves - metabolism; Plant physiology and development; Separation; Solvent mixtures; Solvents; Wavelengths; Solvent effect; Chemical analysis; Phase composition; Metabolite; HPLC chromatography; Extraction yields; Plant leaf; Solvent extraction; Mobile phase; Reversed phase chromatography; Ultraviolet detector; Sample preparation; Metabolites; Dicotyledones; Angiospermae; Plant origin; Annona muricata L. leaves; Mobile phase strength; Spermatophyta; Annonaceae; Solvent mixtures
• ISSN: 1615-9306; 1615-9314
• DOI: 10.1002/jssc.200900375

The effects of five extraction solvents and their mixtures on the yield of metabolites in crude and fractionated extracts of Annona muricata L. leaves were investigated by direct comparison. Extraction media were prepared using simplex centroid mixtures of ethanol, ethyl acetate, dichloromethane, acetone, and chloroform. The effects of the mobile phase solvent strength and the analysis wavelength on the chromatographic separation were also investigated. Solvent mixtures rather than pure solvents were found to be the most efficient extractors for the different fractions. The results indicated that the mobile phase composed of methanol/acetonitrile/water (26:27:47 v/v/v) was most suitable for the basic fraction analysis at 254 nm, whereas the mobile phase composed of methanol/acetonitrile/water (35:35:30 v/v/v) was the most adequate for the organic fraction analysis at 254 nm. The results indicated that the chromatographic profiles and number of peaks were affected by the mobile phase strength and analysis wavelength.