FNIP1 abrogation promotes functional revascularization of ischemic skeletal muscle by driving macrophage recruitment

• Published in: Nature communications Vol. 14; no. 1; p. 7136
• Main Authors: Sun, Zongchao, Yang, Likun, Kiram, Abdukahar, Yang, Jing, Yang, Zhuangzhuang, Xiao, Liwei, Yin, Yujing, Liu, Jing, Mao, Yan, Zhou, Danxia, Yu, Hao, Zhou, Zheng, Xu, Dengqiu, Jia, Yuhuan, Ding, Chenyun, Guo, Qiqi, Wang, Hongwei, Li, Yan, Wang, Li, Fu, Tingting, Hu, Shijun, Gan, Zhenji
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 06-11-2023; Nature Publishing Group; Nature Portfolio
• Subjects: 13; 13/1; 13/31; 13/51; 14/19; 38; 38/47; 38/91; 49/39; 631/443/1338; 631/443/319; 631/80/304; 64/60; Ablation; Angiogenesis; Blood flow; Blood vessels; Chemokines; Humanities and Social Sciences; Ischemia; Limbs; Macrophages; Morbidity; multidisciplinary; Muscles; Musculoskeletal system; Recovery; Science; Science (multidisciplinary); Skeletal muscle; Vascular diseases
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-023-42690-9

Ischaemia of the heart and limbs attributable to compromised blood supply is a major cause of mortality and morbidity. The mechanisms of functional angiogenesis remain poorly understood, however. Here we show that FNIP1 plays a critical role in controlling skeletal muscle functional angiogenesis, a process pivotal for muscle revascularization during ischemia. Muscle FNIP1 expression is down-regulated by exercise. Genetic overexpression of FNIP1 in myofiber causes limited angiogenesis in mice, whereas its myofiber-specific ablation markedly promotes the formation of functional blood vessels. Interestingly, the increased muscle angiogenesis is independent of AMPK but due to enhanced macrophage recruitment in FNIP1-depleted muscles. Mechanistically, myofiber FNIP1 deficiency induces PGC-1α to activate chemokine gene transcription, thereby driving macrophage recruitment and muscle angiogenesis program. Furthermore, in a mouse hindlimb ischemia model of peripheral artery disease, the loss of myofiber FNIP1 significantly improved the recovery of blood flow. Thus, these results reveal a pivotal role of FNIP1 as a negative regulator of functional angiogenesis in muscle, offering insight into potential therapeutic strategies for ischemic diseases.
Functional revascularization is vital to the recovery of blood flow. Here, Sun et al. show that myofiber FNIP1 is a negative regulator of muscle functional angiogenesis and revascularization after ischemia by controlling macrophage recruitment.