Seasonal differences in intestinal flora are related to rats' intestinal water metabolism

• Published in: Frontiers in microbiology Vol. 14; p. 1109696
• Main Authors: Li, Jing, Sun, Yike, Wang, Ruochong, Ma, Shuran, Shi, Lei, Wang, Kai, Zhang, Hairong, Wang, Tong, Liu, Leilei
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 24-02-2023
• Subjects: AQP3; AQP4; AQP8; intestinal flora; intestinal water metabolism; Microbiology; seasonal differences; AQP8; AQP4; seasonal differences; 5-HT; VIP; intestinal water metabolism; AQP3; intestinal flora
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2023.1109696

Many studies have reported obvious seasonal differences in the intestinal flora of rats, and this stable distribution of the seasonal flora helps in maintaining the normal physiological function of the host. However, the mechanism underlying these seasonal differences in intestinal flora remains unclear. To explore the correlation among seasonal factors and intestinal water metabolism and intestinal flora, 20 Sprague Dawley (SD) rats were divided into spring, summer, autumn, and winter groups. The environment for the four seasons was simulated using the Balanced Temperature and Humidity Control system. The intestinal water metabolism was evaluated by determining the intestinal transmission function, fecal water content, water content of colonic tissue, and the colonic expression levels of AQP3, AQP4, and AQP8. The composition and relative abundance of intestinal microflora in rats in each season were assessed through 16S rDNA amplifier sequencing, and the relationship between the dominant flora and intestinal water metabolism in each season was analyzed using Spearman correlation analysis. The high temperature and humidity season could lead to an increase in intestinal water metabolism and intestinal water content in rats, whereas the low temperature and humidity season could lead to a decrease, which was closely related to the change in microflora. To explore the molecular mechanism of seasonal changes in intestinal water metabolism, the concentration of colonic 5-HT, VIP, cAMP, and PKA associated with intestinal water metabolism in rats were also examined. Seasonal changes could affect the concentration of colonic 5-HT and VIP in rats, and then regulate AQPs through cAMP/PKA pathway to affect the intestinal water metabolism. These results suggest that seasonal factors affect the level of intestinal water metabolism in rats and result in seasonal differences in intestinal flora.