Characterization of M. tuberculosis strains from West African patients by spoligotyping

• Published in: Microbes and infection Vol. 1; no. 14; pp. 1189 - 1192
• Main Authors: Niang, Mbayame Ndiaye, Goguet de la Salmoniere, Yves, Samb, Abibou, Hane, Abdoul Almamy, Cisse, Moussa Fafa, Gicquel, Brigitte, Perraut, Ronald
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier SAS 01-12-1999; Amsterdam Elsevier; Paris
• Subjects: Bacterial diseases; Bacterial Typing Techniques; Biological and medical sciences; Cote d'Ivoire - epidemiology; Genome, Bacterial; Genotype; Human bacterial diseases; Humans; Infectious diseases; Medical sciences; Mycobacterium tuberculosis; Mycobacterium tuberculosis - classification; Mycobacterium tuberculosis - genetics; Mycobacterium tuberculosis strains; PCR; Polymerase Chain Reaction; Polymorphism, Genetic - genetics; Senegal; Senegal - epidemiology; spoligotyping; Tuberculosis - epidemiology; Tuberculosis - microbiology; Tuberculosis and atypical mycobacterial infections; West Africa; West Africa; Africa; Senegal; Cote d'Ivoire; spoligotyping; Mycobacterium tuberculosis strains; West Africa; PCR; Human; Respiratory disease; Genotype; Mycobacterial infection; Infection; Characterization; Tuberculosis; Mycobacterium tuberculosis; Mycobacteriales; Bacteriosis; Mycobacteriaceae; Molecular epidemiology; Bacteria; Actinomycetes; Clinical isolate
• ISSN: 1286-4579; 1769-714X
• DOI: 10.1016/S1286-4579(99)00243-9

Analysis of
Mycobacterium tuberculosis strains was carried out using isolates collected from 69 Senegalese and 20 Ivory Coast tuberculosis patients. These 89 isolates were typed by means of the spoligotyping technique, showing clusterized populations of bacterial strains. In the Senegalese patients, 35 genetic profiles were observed with 10 clusters of spoligotypes from 44 isolates. Among Ivory Coast patients, 11 spoligotypes were found for 20 isolates. A particular cluster of isolates was evident both in Senegalese (10) and Ivory Coast (11) patients. These results show the existence of polymorphism of the direct repeat region for African
M. tuberculosis strains. However they suggest that additionnal markers are needed for accurate epidemiological studies in areas that are highly endemic for tuberculosis.