In vivo investigation of ceftiofur-loaded gelatin and PLGA microspheres in beagle dogs

• Published in: Journal of materials science. Materials in medicine Vol. 24; no. 4; pp. 903 - 910
• Main Authors: Hao, Zhihui, Wang, Leilei, Xiao, Kefeng, Zhao, Yongda, Zou, Ming, Zhang, Qidi, Ding, Zhaopeng, Yang, Fenfang, Qu, Baohan
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01-04-2013; Springer; Springer Nature B.V
• Subjects: Animals; Antibacterial agents; Antibiotics. Antiinfectious agents. Antiparasitic agents; Biocompatibility; Biological and medical sciences; Biomaterials; Biomedical Engineering and Bioengineering; Biomedical materials; Cephalosporins - administration & dosage; Ceramics; Chemistry and Materials Science; Chromatography, High Pressure Liquid; Composites; Dogs; Drug delivery systems; Gelatin - administration & dosage; Gelatins; Glass; In vivo testing; In vivo tests; Lactic Acid - administration & dosage; Male; Materials Science; Medical sciences; Microspheres; Natural Materials; Pharmacology. Drug treatments; Polyglycolic Acid - administration & dosage; Polylactic acid; Polymer Sciences; Proteins; Regenerative Medicine/Tissue Engineering; Surfaces and Interfaces; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Surgical implants; Technology. Biomaterials. Equipments; Thin Films; PLGA Microsphere; Gelatin; Carboxymethyl Chitosan; High Performance Liquid Chromatography; Clearance Ratio; Glycolic acid copolymer; Fissipedia; Carnivora; Lactone copolymer; Microsphere; Ceftiofur; β-Lactams; Lactic acid copolymer; In vivo; Cephalosporin derivatives; Vertebrata; Antibiotic; Mammalia; Animal; Animal protein; Antibacterial agent; Dog; Biomedical engineering
• ISSN: 0957-4530; 1573-4838
• DOI: 10.1007/s10856-012-4846-5

Drug delivery systems based on polymer microspheres have received considerable attention. Ceftiofur sodium and ceftiofur hydrochloride is widely used for the treatment of bacterial diseases in animals but the delivery in vivo has not been reported. In this paper, we report the synthesis of microspheres from gelatin and PLGA, two kinds of typical natural and artificial materials, for loading ceftiofur and the in vivo investigation of the pharmacokinetics in beagle dogs. By controlling the synthesis parameters, gelatin and PLGA microspheres with diameter between 5 and 35 microns were obtained. Assay procedures based on high performance liquid chromatography were evaluated and confirmed. The dogs were randomly divided into three groups, i.e., control group, gelatin group, and PLGA group and administrated via intravenous injection. Plasma concentrations of ceftiofur over time were measured and analyzed. Results indicate that the main kinetic parameters do not show significant difference for the gelatin group and control group, but the area under the curve, plasma half-life, apparent volume of distribution, and clearance ratio of PLGA group show significant difference from the gelatin group and the control group. The PLGA microspheres show a low area under the curve but long time release.