Identification of a new probiotic strain, Lactiplantibacillus plantarum VHProbi ® V38, and its use as an oral health agent

• Published in: Frontiers in microbiology Vol. 13; p. 1000309
• Main Authors: Zhang, Jingyan, Duan, Zhi
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 12-12-2022
• Subjects: adhesion; biofilm; caries; Lactiplantibacillus plantarum VHProbi® V38; Microbiology; oral probiotics; periodontal pathogenic bacteria; oral probiotics; adhesion; Lactiplantibacillus plantarum VHProbi® V38; biofilm; caries; periodontal pathogenic bacteria
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2022.1000309

Probiotics can be used to treat oral diseases such as dental caries, gingivitis, periodontitis, and halitosis.
This study screened for strains capable of inhibiting
,one of the primary pathogenic bacteria responsible for dental caries by agar diffusion in different samples. Strain identification was performed by 16S rDNA sequencing and the API 50CH system. The potential functions of the strains in terms of oral health properties were also tested by agglutination assays, growth inhibition assays, adhesion assays, biofilm removal assays and inhibition of adhesion in human primary gingival epithelial (HPGE) cells assays.
This study identified a probiotic strain from fermented cabbages that has a strong inhibitory effect on
. The API 50CH system and 16S rDNA sequencing verified that this was a new strain and it was given the name,
VHProbi
V38. Agglutination, growth inhibition and adhesion, and biofilm removal tests indicated that
VHProbi
V38 inhibited and reduced
. This probiotic was shown to have a broad antibacterial spectrum, simultaneously inhibiting the growth of periodontal pathogenic bacteria such as
,
, and
. After 2 hours of co-cultivation with these pathogens,
VHProbi
V38 was able to significantly reduce pathogens adhesion on human primary gingival epithelial (HPGE) cells.
These findings suggest that
VHProbi
V38 could potentially prevent and treat periodontal diseases caused by these pathogenic bacteria.
VHProbi
V38 also adheres strongly to HPGE cells and thus has potential as an oral probiotic. This study describes new methods that can be used to aid the screening and identification of oral probiotics.