Effect of laser phototherapy on the release of fibroblast growth factors by human gingival fibroblasts

• Published in: Lasers in medical science Vol. 24; no. 6; pp. 885 - 891
• Main Authors: Damante, Carla Andreotti, De Micheli, Giorgio, Miyagi, Sueli Patrícia Harumi, Feist, Ilíria Salomão, Marques, Márcia Martins
• Format: Journal Article
• Language: English
• Published: London Springer-Verlag 01-11-2009; Springer Nature B.V
• Subjects: Animals; Cattle; Cell growth; Cells, Cultured; Culture Media; Dentistry; Fibroblast Growth Factor 2 - biosynthesis; Fibroblast Growth Factor 7 - biosynthesis; Fibroblast Growth Factors - biosynthesis; Fibroblasts - metabolism; Fibroblasts - radiation effects; Gingiva - cytology; Gingiva - metabolism; Gingiva - radiation effects; Gingivoplasty; Humans; Lasers; Low-Level Light Therapy; Medicine; Medicine & Public Health; Optical Devices; Optics; Original Article; Photodynamic therapy; Photonics; Phototherapy; Quantum Optics; Wound healing; Wound Healing - physiology; Wound Healing - radiation effects; Basic fibroblast growth factor; Wound healing; Keratinocyte growth factor
• ISSN: 0268-8921; 1435-604X
• DOI: 10.1007/s10103-008-0582-y

The effects of laser phototherapy on the release of growth factors by human gingival fibroblasts were studied in vitro. Cells from a primary culture were irradiated twice (6 h interval), with continuous diode laser [gallium–aluminum–arsenium (GaAlAs), 780 nm, or indium–gallium–aluminum–phosphide (InGaAlP),_660 nm] in punctual and contact mode, 40 mW, spot size 0.042 cm
2
, 3 J/cm
2
and 5 J/cm
2
(3 s and 5 s, respectively). Positive [10% fetal bovine serum (FBS)] and negative (1%FBS) controls were not irradiated. Production of keratinocyte growth factor (KGF) and basic fibroblast growth factor (bFGF) was quantified by enzyme-linked immunosorbent assay (ELISA). The data were statistically compared by analysis of variance (ANOVA) followed by Tukey’s test (
P
 ≤ 0.05). The characterization of the cell line indicated a mesenchymal nature. KGF release was similar in all groups, while that of bFGF was significantly greater (1.49-times) in groups treated with infra-red laser. It was concluded that increased production of bFGF could be one of the mechanisms by which infra-red laser stimulates wound healing.