MRI/PET multimodal imaging of the innate immune response in skeletal muscle and draining lymph node post vaccination in rats

• Published in: Frontiers in immunology Vol. 13; p. 1081156
• Main Authors: Madi, Saaussan, Xie, Fang, Farhangi, Kamyar, Hsu, Chih-Yang, Cheng, Shih-Hsun, Aweda, Tolulope, Radaram, Bhasker, Slania, Stephanie, Lambert, Tammy, Rambo, Mary, Skedzielewski, Tina, Cole, Austin, Sherina, Valeriia, McKearnan, Shannon, Tran, Hoang, Alsaid, Hasan, Doan, Minh, Stokes, Alan H, O'Hagan, Derek T, Maruggi, Giulietta, Bertholet, Sylvie, Temmerman, Stéphane T, Johnson, Russell, Jucker, Beat M
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 11-01-2023
• Subjects: Animals; AS01; Cytomegalovirus; Cytomegalovirus Infections; Fluorodeoxyglucose F18; Immunity, Innate; Immunology; innate immune activation; lipid nanoparticle; Lymph Nodes - diagnostic imaging; magnetic resonance imaging; Magnetic Resonance Imaging - methods; Multimodal Imaging; Muscle, Skeletal - diagnostic imaging; Positron-Emission Tomography; Rats; self-amplifying mRNA; Vaccination; innate immune activation; lipid nanoparticle; magnetic resonance imaging; self-amplifying mRNA; positron emission tomography; AS01
• ISSN: 1664-3224; 1664-3224
• DOI: 10.3389/fimmu.2022.1081156

The goal of this study was to utilize a multimodal magnetic resonance imaging (MRI) and positron emission tomography (PET) imaging approach to assess the local innate immune response in skeletal muscle and draining lymph node following vaccination in rats using two different vaccine platforms (AS01 adjuvanted protein and lipid nanoparticle (LNP) encapsulated Self-Amplifying mRNA (SAM)). MRI and
FDG PET imaging were performed temporally at baseline, 4, 24, 48, and 72 hr post Prime and Prime-Boost vaccination in hindlimb with Cytomegalovirus (CMV) gB and pentamer proteins formulated with AS01, LNP encapsulated CMV gB protein-encoding SAM (CMV SAM), AS01 or with LNP carrier controls. Both CMV AS01 and CMV SAM resulted in a rapid MRI and PET signal enhancement in hindlimb muscles and draining popliteal lymph node reflecting innate and possibly adaptive immune response. MRI signal enhancement and total
FDG uptake observed in the hindlimb was greater in the CMV SAM vs CMV AS01 group (↑2.3 - 4.3-fold in AUC) and the MRI signal enhancement peak and duration were temporally shifted right in the CMV SAM group following both Prime and Prime-Boost administration. While cytokine profiles were similar among groups, there was good temporal correlation only between IL-6, IL-13, and MRI/PET endpoints. Imaging mass cytometry was performed on lymph node sections at 72 hr post Prime and Prime-Boost vaccination to characterize the innate and adaptive immune cell signatures. Cell proximity analysis indicated that each follicular dendritic cell interacted with more follicular B cells in the CMV AS01 than in the CMV SAM group, supporting the stronger humoral immune response observed in the CMV AS01 group. A strong correlation between lymph node MRI T2 value and nearest-neighbor analysis of follicular dendritic cell and follicular B cells was observed (r=0.808, P<0.01). These data suggest that spatiotemporal imaging data together with AI/ML approaches may help establish whether
imaging biomarkers can predict local and systemic immune responses following vaccination.