Cross-resistance study and biochemical mechanisms of thiamethoxam resistance in B-biotype Bemisia tabaci (Hemiptera: Aleyrodidae)

• Published in: Pest management science Vol. 66; no. 3; pp. 313 - 318
• Main Authors: Feng, Yuntao, Wu, Qingjun, Wang, Shaoli, Chang, Xiaoli, Xie, Wen, Xu, Baoyun, Zhang, Youjun
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01-03-2010; Wiley; Wiley Subscription Services, Inc
• Subjects: abamectin; acetamiprid; Aleyrodidae; Animals; artificial selection; Bemisia tabaci; Biological and medical sciences; biotypes; carbosulfan; carboxylesterase; Chemical compounds; chlorpyrifos; Control; cross resistance; cytochrome P-450; defense mechanisms; deltamethrin; Drug Synergism; enzyme activity; Female; fipronil; Fundamental and applied biological sciences. Psychology; glutathione transferase; Hemiptera; Hemiptera - classification; Hemiptera - drug effects; Hemiptera - enzymology; Hemiptera - metabolism; imidacloprid; Insect control; insect pests; Insecticide Resistance; Insecticides; Insecticides - pharmacology; Male; metabolic detoxification; Neonicotinoids; nitenpyram; Nitro Compounds - pharmacology; Oxazines - pharmacology; oxidase; pesticide synergists; Phytopathology. Animal pests. Plant and forest protection; piperonyl butoxide; plant pests; Protozoa. Invertebrates; resistance mechanisms; Resistance to control; thiamethoxam; Thiazoles - pharmacology; triphenyl phosphate; China; Insecticide; Enzyme; Insecta; Pesticides; Oxidase; thiamethoxam; Guanidines; Biochemistry; resistance mechanisms; Neonicotinoid; Pest; Homoptera; Arthropoda; Bemisia tabaci; Biotype; Aleyrodidae; Cross resistance; cross-resistance; Oxidoreductases; Invertebrata
• ISSN: 1526-498X; 1526-4998; 1526-4998
• DOI: 10.1002/ps.1877

BACKGROUND: B-biotype Bemisia tabaci (Gennadius) has invaded China over the past two decades. To understand the risks and to determine possible mechanisms of resistance to thiamethoxam in B. tabaci, a resistant strain was selected in the laboratory. Cross-resistance and the biochemical mechanisms of thiamethoxam resistance were investigated in the present study.RESULTS: A 66.3-fold thiamethoxam-resistant B. tabaci strain (TH-R) was established after selection for 36 generations. Compared with the susceptible strain (TH-S), the selected TH-R strain showed obvious cross-resistance to imidacloprid (47.3-fold), acetamiprid (35.8-fold), nitenpyram (9.99-fold), abamectin (5.33-fold) and carbosulfan (4.43-fold). No cross-resistance to fipronil, chlorpyrifos or deltamethrin was seen. Piperonyl butoxide (PBO) and triphenyl phosphate (TPP) exhibited significant synergism on thiamethoxam effects in the TH-R strain (3.14- and 2.37-fold respectively). However, diethyl maleate (DEM) did not act synergistically with thiamethoxam. Biochemical assays showed that cytochrome P450 monooxygenase activities increased 1.21- and 1.68-fold respectively, and carboxylesterase activity increased 2.96-fold in the TH-R strain. However, no difference was observed for glutathione S-transferase between the two strains.CONCLUSION: B-biotype B. tabaci develops resistance to thiamethoxam. Cytochrome P450 monooxygenase and carboxylesterase appear to be responsible for the resistance. Reasonable resistance management that avoids the use of cross-resistance insecticides may delay the development of resistance to thiamethoxam in this species.