Enzymatic glycerolysis and transesterification of vegetable oil for enhanced production of feruloylated glycerols

• Published in: Journal of the American Oil Chemists' Society Vol. 83; no. 9; pp. 765 - 770
• Main Authors: Laszlo, Joseph A, Compton, David L
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01-09-2006; Springer‐Verlag; Springer; Springer Nature B.V
• Subjects: Biological and medical sciences; diacylglycerols; Diglycerides; Enzymatic transesterification; Enzymes; Fat industries; ferulic acid; Food industries; Functional groups; Fundamental and applied biological sciences. Psychology; glycerolysis; Glycine max; Hydroxyl groups; lipase; monoacylglycerols; Reaction kinetics; soybean oil; Transesterification; triacylglycerol lipase; triacylglycerols; triglyceride; vegetable oil; Vegetable oils; Vegetables; Water activity; Enzyme; Phenolic acid; lipase; Glycerol; Triacylglycerol lipase; Lipids; Esterases; Vegetable oil; Transesterification; Triglyceride; Cinnamic acid(4-hydroxy 3-methoxy); Carboxylic ester hydrolases; Oils and fats industry; Enzymatic transesterification; ferulic acid; Production; Hydrolases; Enzymatic reaction
• ISSN: 0003-021X; 1558-9331
• DOI: 10.1007/s11746-006-5012-3

Novel functional groups can be introduced into vegetable oils using enzymes, resulting in value-added products. The transesterification kinetics of ethyl ferulate with MAG, DAG, and TAG were examined. Transesterification was catalyzed by immobilized Candida antarctica lipase B in solventless batch and packed-bed reactors. Initial reaction rates with TAG were slightly sensitive to water activity, whereas rates with MAG and DAG were water activity independent. Transesterification was also three-to sixfold faster with MAG and DAG. These observations indicate that the reaction is rate limited by the acyl acceptor, and that oils with free hydroxyl groups are preferred acyl acceptors in comparison with TAG, which must undergo partial hydrolysis before becoming reactive.