Sphingobium vermicomposti sp. nov., isolated from vermicompost

• Published in: International journal of systematic and evolutionary microbiology Vol. 59; no. 12; pp. 3145 - 3149
• Main Authors: Vaz-Moreira, Ivone, Faria, Catia, Lopes, Ana R, Svensson, Liselott, Falsen, Enevold, Moore, Edward R. B, Ferreira, Antonio C. Silva, Nunes, Olga C, Manaia, Celia M
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01-12-2009; Society for General Microbiology
• Subjects: Bacteriology; Biological and medical sciences; Biological Systematics; Biologisk systematik; DNA, Bacterial - genetics; DNA, Ribosomal - genetics; Fatty Acids - chemistry; Fatty Acids - metabolism; Fundamental and applied biological sciences. Psychology; Microbiology; Molecular Sequence Data; Phylogeny; RNA, Ribosomal, 16S - genetics; Soil - analysis; Soil Microbiology; Sphingobium; Sphingomonadaceae - classification; Sphingomonadaceae - genetics; Sphingomonadaceae - isolation & purification; Sphingomonadaceae - metabolism; Systematics; Taxonomy; Bacteria; New species
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.006163-0

1 Escola Superior de Biotecnologia, Universidade Católica Portuguesa, 4200-072 Porto, Portugal
2 LEPAE – Departamento de Engenharia Química, Faculdade de Engenharia, Universidade do Porto, 4200-465 Porto, Portugal
3 CCUG – Culture Collection University Göteborg, Sahlgrenska University Hospital, Sahlgrenska Academy, University of Göteborg, S-41346 Göteborg, Sweden
Correspondence Célia M. Manaia cmmanaia{at}esb.ucp.pt
Strain VC-230 T was isolated from homemade vermicompost produced from kitchen waste. The isolate was a Gram-negative-staining, catalase- and oxidase-positive, motile rod-shaped bacterium able to grow at 15–37 °C and pH 6–8. On the basis of 16S rRNA gene sequence analysis, strain VC-230 T was determined to belong to the family Sphingomonadaceae by its clustering with type strains of the genus Sphingobium , with Sphingobium chlorophenolicum ATCC 33790 T (97.7 %) and Sphingobium herbicidovorans DSM 11019 T (97.4 %) as its closest neighbours. The polar lipid pattern, the presence of spermidine and ubiquinone 10, the predominance of the cellular fatty acids C 18 : 1 7 c /9 t /12 t , C 16 : 1 7 c and C 16 : 0 and the G+C content of the genomic DNA supported the affiliation of this organism to the genus Sphingobium . The phylogenetic, chemotaxonomic, phenotypic and DNA–DNA hybridization analyses verify that strain VC-230 T represents a novel species, for which the name Sphingobium vermicomposti sp. nov. is proposed. The type strain is VC-230 T (=CCUG 55809 T =DSM 21299 T ).
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain VC-230 T is AM998824 .