A plant polyphenol-rich extract restores the suppressed functions of phagocytes in influenza virus-infected mice

• Published in: Microbes and infection Vol. 7; no. 3; pp. 391 - 398
• Main Authors: Ivanova, Emilia, Toshkova, Reneta, Serkedjieva, Julia
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier SAS 01-03-2005; Amsterdam Elsevier; Paris
• Subjects: Animals; Biological and medical sciences; Female; Fundamental and applied biological sciences. Psychology; General pharmacology; Geraniaceae - chemistry; Geranium sanguineum; Influenza virus; Influenza virus infection; Macrophages; Male; Medical sciences; Mice; Mice, Inbred ICR; Microbiology; Miscellaneous; Orthomyxoviridae Infections - drug therapy; Orthomyxoviridae Infections - immunology; Phagocytes - drug effects; Phagocytes - virology; Phagocytosis; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant polyphenols; Virology; PBS; SS; cRPMI; CH; PMNs; i.n; p.i; Macrophages; aMØ; VC; PC; LAI; TCID 50; PI; pMØ; VIM; Plant polyphenols; Phagocytosis; Influenza virus infection; Rodentia; Orthomyxoviridae; Geraniaceae; Medicinal plant; Virus; Infection; Plant; Vertebrata; Polyphenol; Mammalia; Influenzavirus A; Mouse; Dicotyledones; Viral disease; Angiospermae; Phenols; Spermatophyta; Geranium; Macrophage
• ISSN: 1286-4579; 1769-714X
• DOI: 10.1016/j.micinf.2004.11.013

Influenza infection was induced in white ICR mice by intranasal (i.n.) inoculation of the virus A/Aichi/2/68 (H3N2). The number, migration and phagocyte indices of alveolar and peritoneal macrophages (pMØ) and of blood polymorphonuclear leukocytes (PMNs), as well as the inhibition of the PMN adherence in the presence of a specific antigen were followed for 9 days after infection. The effect of the i.n. application of a polyphenol-rich extract, designated as polyphenolic complex (PC), isolated from the medicinal plant
Geranium sanguineum L., on the inspected immune parameters was studied in parallel with the virological parameters of the infection, e.g. rate of mortality, mean survival time (MST), infectious lung virus titre and consolidation of the lungs. It was found that the application of PC induced a continuous 2- to 2.5-fold rise in the number of both peritoneal and alveolar macrophages (aMØ) in the infected and healthy controls. The migration of both peritoneal and aMØ increased 1.5- to 2-fold in the group of infected PC-treated animals and four to fivefold in the control group, the maximum being on day 9. PC stimulated phagocyte activities of blood PMNs in both infected and healthy mice. The leukocyte adherence inhibition (LAI) index decreased in the infected and PC-treated animals. The restoration of the suppressed functions of phagocytes in influenza virus-infected mice (VIM) was consistent with a prolongation of MST and reduction in mortality rate, infectious virus titre and lung consolidation. The immunoenhancing properties of PC apparently contribute to the overall protective effect of the plant preparation in the lethal murine experimental influenza A/Aichi infection.