Polyethylene, whose surface has been modified by UV irradiation, induces cytotoxicity: A comparison with microplastics found in beaches

• Published in: Ecotoxicology and environmental safety Vol. 277; p. 116346
• Main Authors: Ikuno, Yudai, Tsujino, Hirofumi, Haga, Yuya, Manabe, Sota, Idehara, Wakaba, Hokaku, Mii, Asahara, Haruyasu, Higashisaka, Kazuma, Tsutsumi, Yasuo
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01-06-2024; Elsevier
• Subjects: Animals; Bathing Beaches; Cell Line; Cell Survival - drug effects; Cytotoxicity; Humans; Microplastic degradation; Microplastics; Microplastics - toxicity; Plastics - toxicity; Polyethylene; Polyethylene - chemistry; Polyethylene - toxicity; Ultraviolet Rays; Vacuum ultraviolet irradiation; Water Pollutants, Chemical - toxicity; Polyethylene; PS; ATR-IR; Cytotoxicity; MTT; Vacuum ultraviolet irradiation; PE; VUV; FBS; IC50; CMC; Microplastic degradation; Microplastics
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2024.116346

Microplastics, plastic particles 5 mm or less in size, are abundant in the environment; hence, the exposure of humans to microplastics is a great concern. Usually, the surface of microplastics found in the environment has undergone degradation by external factors such as ultraviolet rays and water waves. One of the characteristics of changes caused by surface degradation of microplastics is the introduction of oxygen-containing functional groups. Surface degradation alters the physicochemical properties of plastics, suggesting that the biological effects of environmentally degraded plastics may differ from those of pure plastics. However, the biological effects of plastics introduced with oxygen-containing functional groups through degradation are poorly elucidated owing to the lack of a plastic sample that imitates the degradation state of plastics found in the environment. In this study, we investigated the degradation state of microplastics collected from a beach. Next, we degraded a commercially available polyethylene (PE) particles via vacuum ultraviolet (VUV) irradiation and showed that chemical surface state of PE imitates that of microplastics in the environment. We evaluated the cytotoxic effects of degraded PE samples on immune and epithelial cell lines. We found that VUV irradiation was effective in degrading PE within a short period, and concentration-dependent cytotoxicity was induced by degraded PE in all cell lines. Our results indicate that the cytotoxic effect of PE on different cell types depends on the degree of microplastic degradation, which contributes to our understanding of the effects of PE microplastics on humans.
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•Surface of microplastics in the environment was chemically degraded.•Vacuum ultraviolet irradiation was performed to chemically degrade polyethylene (PE).•Experimentally degraded PE product imitated the environmentally degraded PE.•Degraded PE microplastics induced cytotoxicity to immune and epithelial cells.