Fatty acid unsaturation improves germination of upland cotton ( Gossypium hirsutum ) under cold stress

• Published in: Frontiers in plant science Vol. 15; p. 1286908
• Main Authors: Dhaliwal, Lakhvir Kaur, Shim, Junghyun, Auld, Dick, Angeles-Shim, Rosalyn B
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 06-02-2024
• Subjects: cell membrane; cold stress; cumulative melting temperature; imbibition; Plant Science; seed germination; unsaturated fatty acids; unsaturated fatty acids; cell membrane; cold stress; imbibition; seed germination; cumulative melting temperature; recombinant inbred lines
• ISSN: 1664-462X; 1664-462X
• DOI: 10.3389/fpls.2024.1286908

The level of fatty acid unsaturation in seeds is one of the major determinants of cold germination ability, particularly in oilseeds. The presence of
double bonds in unsaturated fatty acids creates bends that lowers their melting temperatures compared to saturated fatty acids. Unsaturated fatty acids with low melting points mobilize faster at low temperatures providing seeds with sufficient energy for germination.
To investigate the effects of fatty acid unsaturation on the ability of cotton seeds to germinate under cold conditions, four recombinant inbred lines (RILs) of cotton with unique fatty acid profiles were evaluated using a set of developmental and biochemical assays at 12°C (critically low temperature), 15°C (cardinal minimum temperature) and 30°C (optimum temperature). Furthermore, whole seed lipidome profiling using liquid chromatography with mass spectrometry was done to compare the lipid compositional changes at 12°C and 30°C after imbibing cotton seeds of all the six genotypes for 0 hours, 3 hours and 6 hours.
The RILs with higher unsaturation/saturation ratios registered robust germination performance, lower solute leakage, and optimum water uptake rates under cold stress. Imbibition at 30°C for 8 hours before cold exposure significantly improved the germination of cold sensitive genotypes, indicating that the first few hours of water uptake are critical for cold stress. Whole seed lipidome profiling of all the genotypes specifically associated cold germination ability with higher unsaturation levels of phospholipids during early imbibition. The presence of
double bonds in phospholipids creates kinks that maintain the fluidity of cell membranes under low temperature. Membrane flexibility under cold conditions is essential for facilitating key germination events including membrane organization and respiration. The current results highlight the importance of fatty acid composition in cold germination ability of upland cotton.