A Transverse Tubule NADPH Oxidase Activity Stimulates Calcium Release from Isolated Triads via Ryanodine Receptor Type 1 S -Glutathionylation

• Published in: The Journal of biological chemistry Vol. 281; no. 36; pp. 26473 - 26482
• Main Authors: Hidalgo, Cecilia, Sánchez, Gina, Barrientos, Genaro, Aracena-Parks, Paula
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 08-09-2006; American Society for Biochemistry and Molecular Biology
• Subjects: Animals; Calcium - metabolism; Calcium Channels, L-Type - metabolism; Glutathione - metabolism; Hydrogen Peroxide - metabolism; Mice; Muscle Fibers, Skeletal - enzymology; Muscle Fibers, Skeletal - metabolism; Muscle Fibers, Skeletal - ultrastructure; NAD - metabolism; NADP - metabolism; NADPH Oxidases - antagonists & inhibitors; NADPH Oxidases - metabolism; Oxidants - metabolism; Protein Isoforms - metabolism; Protein Subunits - metabolism; Rabbits; Ryanodine Receptor Calcium Release Channel - metabolism; Sarcoplasmic Reticulum - metabolism; Sarcoplasmic Reticulum - ultrastructure
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M600451200

We report here the presence of an NADPH oxidase (NOX) activity both in intact and in isolated transverse tubules and in triads isolated from mammalian skeletal muscle, as established by immunochemical, enzymatic, and pharmacological criteria. Immunohistochemical determinations with NOX antibodies showed that the gp91phox membrane subunit and the cytoplasmic regulatory p47phox subunit co-localized in transverse tubules of adult mice fibers with the α1s subunit of dihydropyridine receptors. Western blot analysis revealed that isolated triads contained the integral membrane subunits gp91phox and p22phox, which were markedly enriched in isolated transverse tubules but absent from junctional sarcoplasmic reticulum vesicles. Isolated triads and transverse tubules, but not junctional sarcoplasmic reticulum, also contained varying amounts of the cytoplasmic NOX regulatory subunits p47phox and p67phox. NADPH or NADH elicited superoxide anion and hydrogen peroxide generation by isolated triads; both activities were inhibited by NOX inhibitors but not by rotenone. NADH diminished the total thiol content of triads by one-third; catalase or apocynin, a NOX inhibitor, prevented this effect. NADPH enhanced the activity of ryanodine receptor type 1 (RyR1) in triads, measured through [3H]ryanodine binding and calcium release kinetics, and increased significantly RyR1 S-glutathionylation over basal levels. Preincubation with reducing agents or NOX inhibitors abolished the enhancement of RyR1 activity produced by NADPH and prevented NADPH-induced RyR1 S-glutathionylation. We propose that reactive oxygen species generated by the transverse tubule NOX activate via redox modification the neighboring RyR1 Ca2+ release channels. Possible implications of this putative mechanism for skeletal muscle function are discussed.