Met-enkephalin induces cytolytic processes of apoptotic type in K562 human erythroid leukemia cells

• Published in: FEBS letters Vol. 383; no. 3; pp. 230 - 232
• Main Authors: Mernenko, Olga A., Blishchenko, Elena Yu, Mirkina, Irina I., Karelin, Andrey A.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 01-04-1996
• Subjects: Apoptosis; Apoptosis - drug effects; Cell Line; Cell Survival - drug effects; Cytotoxicity; DNA, Neoplasm - drug effects; DNA, Neoplasm - isolation & purification; Dose-Response Relationship, Drug; Enkephalin, Methionine - pharmacology; Humans; Leukemia, Erythroblastic, Acute; Met-enkephalin; NT II, neurotoxin II from Naja naja oxiana cobra venom; Reproducibility of Results; TNF, tumour necrosis factor; Trypan Blue; Tumor Cells, Cultured; Met-enkephalin; Cytotoxicity; NT II, neurotoxin II from Naja naja oxiana cobra venom; TNF, tumour necrosis factor; Apoptosis
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(96)00208-6

Cytolytic activity of Met-enkephalin, an endogenous opioid peptide, was studied within the 10
−7−10
−17 M concentration range in K562 human erythroid leukemia cells. Cytolytic activity was determined by the trypan blue inclusion method after 13, 15 and 18 h of Met-enkephalin co-incubation with target cells. Discrete maxima of cytolytic activity were detected at concentrations of 10
−9−10
−10, 10
−13 and 10
−15 M. Cytolysis was accompanied by internucleosomal DNA fragmentation which is indicative of the mechanism of cell death being apoptosis.