Methane-Derived Carbon as a Driver for Cyanobacterial Growth

• Published in: Frontiers in microbiology Vol. 13; p. 837198
• Main Authors: Cerbin, Slawek, Pérez, Germán, Rybak, Michał, Wejnerowski, Łukasz, Konowalczyk, Adam, Helmsing, Nico, Naus-Wiezer, Suzanne, Meima-Franke, Marion, Pytlak, Łukasz, Raaijmakers, Ciska, Nowak, Witold, Bodelier, Paul L E
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 01-04-2022
• Subjects: co-culture; greenhouse gases; isotopes; lakes; methane; methane oxidation; Microbiology; greenhouse gases; isotopes; cyanobacteria; methane; lakes; co-culture; methane oxidation
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2022.837198

Methane, a potent greenhouse gas produced in freshwater ecosystems, can be used by methane-oxidizing bacteria (MOB) and can therefore subsidize the pelagic food web with energy and carbon. Consortia of MOB and photoautotrophs have been described in aquatic ecosystems and MOB can benefit from photoautotrophs which produce oxygen, thereby enhancing CH
oxidation. Methane oxidation can account for accumulation of inorganic carbon (i.e., CO
) and the release of exometabolites that may both be important factors influencing the structure of phytoplankton communities. The consortium of MOB and phototroph has been mainly studied for methane-removing biotechnologies, but there is still little information on the role of these interactions in freshwater ecosystems especially in the context of cyanobacterial growth and bloom development. We hypothesized that MOB could be an alternative C source to support cyanobacterial growth in freshwater systems. We detected low δ
C values in cyanobacterial blooms (the lowest detected value -59.97‰ for
) what could be the result of the use of methane-derived carbon by cyanobacteria and/or MOB attached to their cells. We further proved the presence of metabolically active MOB on cyanobacterial filaments using the fluorescein isothiocyanate (FITC) based activity assay. The PCR results also proved the presence of the
gene in several non-axenic cultures of cyanobacteria. Finally, experiments comprising the co-culture of the cyanobacterium
with the methanotroph
proved that cyanobacterial growth was significantly improved in the presence of MOB, presumably through utilizing CO
released by MOB. On the other hand,
C-CH
labeled incubations showed the uptake and assimilation of MOB-derived metabolites by the cyanobacterium. We also observed a higher growth of MOB in the presence of cyanobacteria under a higher irradiance regime, then when grown alone, underpinning the bidirectional influence with as of yet unknown environmental consequences.