CircRNA_100395 Carried by Exosomes From Adipose-Derived Mesenchymal Stem Cells Inhibits the Malignant Transformation of Non-Small Cell Lung Carcinoma Through the miR-141-3p-LATS2 Axis

• Published in: Frontiers in cell and developmental biology Vol. 9; p. 663147
• Main Authors: Zhang, Chong, Cao, Jinlin, Lv, Wang, Mou, Haibo
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 25-03-2021
• Subjects: Cell and Developmental Biology; circRNA_100395; exosomes from adipose-derived mesenchymal stem cells; Hippo/YAP signalling pathway; miR-141-3p; non-small cell lung carcinoma (NSCLC); non-small cell lung carcinoma (NSCLC); miR-141-3p; circRNA_100395; Hippo/YAP signalling pathway; exosomes from adipose-derived mesenchymal stem cells
• ISSN: 2296-634X; 2296-634X
• DOI: 10.3389/fcell.2021.663147

The specific purpose of this study is to investigate the impact exosomes from adipose-derived mesenchymal stem cell (AMSC) has on non-small cell lung carcinoma (NSCLC) and the relative applications.
circ_100395, miR-141-3p, and LATS2 were expressed and detected in NSCLC and paracancerous tissues as well as NSCLC cell lines. Pearson correlation analysis, Dual-Luciferase Reporter Assay and RNA pull-down assay were used to validate their expression and interaction, respectively. After isolation and culture of AMSCs, exosomes were extracted and identified. EdU, epithelial-mesenchymal transition (EMT), and cell colony formation assay were used to distinguish the biological activity of the cells. Expression Hippo/YAP signalling pathway-related proteins were measured by western blotting. Subsequently, tumour volume and weight were confirmed based on xenograft nude mice models, Ki-67 and LATS2 expression was observed by immunohistochemistry.
circ_100395 was lowly expressed in NSCLC tissues or cells. The negative correlations and interactions were confirmed between circ_100395 and miR-141-3p, miR-141-3p, and LATS2. AMSC-derived exosomes with overexpression of circ_100395 (exo-circ_100395) significantly inhibited the biological activity as well as EMT of H1650 cells and Hippo/YAP signalling pathway activity. In addition, exo-circ_100395 markedly reduced tumour volume and weight as well as Ki-67 and LASP1 expression
. However, overexpressed miR-141-3p or knocked down LATS2 alleviated the above effects.
Exo-circ_100395 can increase LATS2 expression by sponging miR-141-3p to regulate Hippo/YAP signalling pathway, thereby inhibiting NSCLC malignant transformation.