Complete Genome Sequencing of a Novel Newcastle Disease Virus Isolate Circulating in Layer Chickens in the Dominican Republic

• Published in: Journal of Virology Vol. 86; no. 17; p. 9550
• Main Authors: Courtney, Sean C, Gomez, Dejelia, Susta, Leonardo, Hines, Nichole, Pedersen, Janice C, Miller, Patti J, Afonso, Claudio L
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01-09-2012
• Subjects: amino acid sequences; Animals; Base Sequence; Biological and medical sciences; Chickens; disease outbreaks; Dominican Republic; Fundamental and applied biological sciences. Psychology; genome; Genome Announcements; Genome, Viral; genotype; hemagglutinins; laying hens; Microbiology; Miscellaneous; Molecular Sequence Data; mortality; Newcastle disease; Newcastle Disease - virology; Newcastle disease virus; Newcastle disease virus - classification; Newcastle disease virus - genetics; Newcastle disease virus - isolation & purification; nucleotide sequences; Poultry Diseases - virology; sialidase; viral fusion proteins; Virology; Dominican Republic; Virus; Paramyxovirinae; Mononegavirales; Vertebrata; Rubulavirus; Isolate; Chicken; Newcastle disease virus; Aves; Genome; Veterinary; Paramyxoviridae
• ISSN: 0022-538X; 1098-5514
• DOI: 10.1128/JVI.01491-12

Newcastle disease virus (NDV) was isolated from an outbreak in layer chickens in the Dominican Republic in 2008. Infections with this isolate led to a 100% apparent case fatality rate in birds. Complete genome sequencing revealed that the isolate does not belong to any of the previously described NDV genotypes. Similarly, large differences were observed in the amino acid sequence of the fusion and hemagglutinin-neuraminidase proteins in comparison with all known NDV genotypes, suggesting the existence of an unknown reservoir for NDV. The work presented here represents the first complete genome sequence of NDV in the Dominican Republic.