Chloroform Extract of Rasagenthi Mezhugu, a Siddha Formulation, as an Evidence-Based Complementary and Alternative Medicine for HPV-Positive Cervical Cancers

• Published in: Evidence-based complementary and alternative medicine Vol. 2012; no. 2012; pp. 1 - 10
• Main Authors: Alshatwi, Ali A., Paul, Preethy, Periasamy, Vaiyapuri S., Riyasdeen, Anvarbatcha, Akbarsha, Mohammad A.
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01-01-2012; Hindawi Limited
• Subjects: Alternative medicine; Apoptosis; Ayurvedic medicine; Cancer therapies; Cell cycle; Cervical cancer; Cervix; Chloroform; DNA damage; Drugs; Genomes; Heavy metals; Herbal medicine; Human papillomavirus; Medical research; Mitochondrial DNA; Traditional medicine; Viability; Zingiber officinale; United States > US; India
• ISSN: 1741-427X; 1741-4288
• DOI: 10.1155/2012/136527

Rasagenthi Mezhugu (RGM) is a herbomineral formulation in the Siddha system of traditional medicine and is prescribed in the southern parts of India as a remedy for all kinds of cancers. However, scientific evidence for its therapeutic efficacy in cervical cancer is lacking, and it contains heavy metals. To overcome these limitations, RGM was extracted, and the fractions were tested on HPV-positive cervical cancer cells, ME-180 and SiHa. The extracts, free from the toxic heavy metals, affected the viability of both the cells. The chloroform fraction (cRGM) induced DNA damage and apoptosis. Mitochondria-mediated apoptosis was indicated. Though both the cells responded to the treatment, ME-180 was more responsive. Thus, this study brings up scientific evidence for the efficacy of RGM against the HPV-mediated cervical cancer cells and, if the toxic heavy metals are the limitation in its use, cRGM would be a suitable candidate as evidence-based complementary and alternative medicine for HPV-positive cervical cancers.