Exploration of the Molecular Mechanisms Underlying the Anti-Photoaging Effect of Limosilactobacillus fermentum XJC60

• Published in: Frontiers in cellular and infection microbiology Vol. 12; p. 838060
• Main Authors: Chen, Huizhen, Li, Ying, Xie, Xinqiang, Chen, Moutong, Xue, Liang, Wang, Juan, Ye, Qinghua, Wu, Shi, Yang, Runshi, Zhao, Hui, Zhang, Jumei, Ding, Yu, Wu, Qingping
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 29-04-2022
• Subjects: anti-photoaging; Cellular and Infection Microbiology; Limosilactobacillus fermentum; nicotinamide; pan-genome analysis; skin; skin; UV; anti-photoaging; Limosilactobacillus fermentum; pan-genome analysis; nicotinamide
• ISSN: 2235-2988; 2235-2988
• DOI: 10.3389/fcimb.2022.838060

Although lactic acid bacteria (LAB) were shown to be effective for preventing photoaging, the underlying molecular mechanisms have not been fully elucidated. Accordingly, we examined the anti-photoaging potential of 206 LAB isolates and discovered 32 strains with protective activities against UV-induced injury. All of these 32 LABs exhibited high levels of 2,2-diphenyl-picrylhydrazyl, as well as hydroxyl free radical scavenging ability (46.89-85.13% and 44.29-95.97%, respectively). Genome mining and metabonomic verification of the most effective strain,
XJC60, revealed that the anti-photoaging metabolite of LAB was nicotinamide (NAM; 18.50 mg/L in the cell-free serum of XJC60). Further analysis revealed that LAB-derived NAM could reduce reactive oxygen species levels by 70%, stabilize the mitochondrial membrane potential, and increase the NAD
/NADH ratio in UV-injured skin cells. Furthermore, LAB-derived NAM downregulated the transcript levels of matrix metalloproteinase (
)-
,
, interleukin (
)-
,
, and
in skin cells.
, XJC60 relieved imflammation and protected skin collagen fiber integrity in UV-injured Guinea pigs. Overall, our findings elucidate that LAB-derived NAM might protect skin from photoaging by stabilizing mitochondrial function, establishing a therotical foundation for the use of probiotics in the maintenance of skin health.