AcBBX5, a B-box transcription factor from pineapple, regulates flowering time and floral organ development in plants

• Published in: Frontiers in plant science Vol. 13; p. 1060276
• Main Authors: Ouyang, Yanwei, Zhang, Xiumei, Wei, Yongzan, He, Yukun, Zhang, Xiaohan, Li, Ziqiong, Wang, Can, Zhang, Hongna
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 24-11-2022
• Subjects: B-box; expression profiling; floral organ; flowering; pineapple (Ananas comosus (L.) Merr.); Plant Science; B-box; floral organ; flowering; pineapple (Ananas comosus (L.) Merr.); expression profiling
• ISSN: 1664-462X; 1664-462X
• DOI: 10.3389/fpls.2022.1060276

Flowering is an important factor to ensure the success of plant reproduction, and reasonable flowering time is crucial to the crop yield. BBX transcription factors can regulate several growth and development processes. However, there is little research on whether BBX is involved in flower formation and floral organ development of pineapple. In this study,
, a BBX family gene with two conserved B-box domains, was identified from pineapple. Subcellular localization analysis showed that AcBBX5 was located in the nucleus. Transactivation analysis indicated that AcBBX5 had no significant toxic effects on the yeast system and presented transcriptional activation activity in yeast. Overexpression of
delayed flowering time and enlarged flower morphology in Arabidopsis. Meanwhile, the expression levels of
,
,
and
were decreased, and the transcription levels of
and
were increased in
-overexpressing Arabidopsis, which might lead to delayed flowering of transgenic plants. Furthermore, transcriptome data and QRT-PCR results showed that
was expressed in all floral organs, with the high expression levels in stamens, ovaries and petals. Yeast one-hybrid and dual luciferase assay results showed that AcBBX5 bound to
promoter and inhibited
gene expression. In conclusion,
was involved in flower bud differentiation and floral organ development, which provides an important reference for studying the functions of BBX and the molecular regulation of flower.