In Vivo Effect of Resveratrol-Loaded Solid Lipid Nanoparticles to Relieve Physical Fatigue for Sports Nutrition Supplements

• Published in: Molecules (Basel, Switzerland) Vol. 25; no. 22; p. 5302
• Main Authors: Qin, Lili, Lu, Tianfeng, Qin, Yao, He, Yiwei, Cui, Ningxin, Du, Ai, Sun, Jingyu
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI 13-11-2020; MDPI AG
• Subjects: Animals; anti-fatigue; Antioxidants - chemistry; Biological Availability; Calorimetry, Differential Scanning; Dietary Supplements; Drug Carriers - chemistry; drug delivery; Fatigue - prevention & control; Lipid Peroxidation; Lipids - chemistry; Male; Mice; Mice, Inbred C57BL; Microscopy, Electron, Transmission; Microtubule-Associated Proteins - metabolism; Nanoparticles - chemistry; nutrition supplements; Particle Size; Physical Conditioning, Animal; resveratrol; Resveratrol - chemistry; Sequestosome-1 Protein - metabolism; Sirtuin 1 - metabolism; solid lipid nanoparticles (SLNs); Solubility; Spectrum Analysis, Raman; Sports Nutritional Physiological Phenomena; solid lipid nanoparticles (SLNs); anti-fatigue; drug delivery; resveratrol; nutrition supplements
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules25225302

Resveratrol (RSV) is a natural flavonoid polyphenol compound extracted from the plants which shows various biological activities. However, the clinical application of RSV is limited by its poor aqueous solubility, rapid metabolism and poor bioavailability. In this study, resveratrol-loaded solid lipid nanoparticles (RSV- SLNs) was design as a nano-antioxidant against the physical fatigue. The resultant RSV-SLNs were characterized by photon correlation spectroscopy (PCS), transmission electron micrographs (TEM), zeta potential, differential scanning calorimetry (DSC) and Raman spectroscopy pattern. Furthermore, the in vivo anti-fatigue effect assays showed that RSV-SLNs prolonged the mice exhausted time and running distance. The biochemical parameters of blood related to fatigue suggested that RSV-SLNs have potential applications to improve the antioxidant defense of the mice after extensive exercise and confer anti-fatigue capability. Furthermore, the molecular mechanisms of antioxidant by RSV-SLNs supplementation was investigated through the analysis of silent information regulator 2 homolog 1 (SIRT1) protein expression, which demonstrated that it could downregulate the expression of SIRT1 and increase autophagy markers, microtubule-associated protein 1 light chain 3-II (LC3-II) and sequestosome-1 (SQSTM1/p62). These results reveal that the RSV-SLNs may have great potential used as a novel anti-fatigue sports nutritional supplement.