The Genomes of Two Strains of Taenia crassiceps the Animal Model for the Study of Human Cysticercosis

• Published in: Frontiers in cellular and infection microbiology Vol. 12; p. 876839
• Main Authors: Bobes, Raúl J, Estrada, Karel, Rios-Valencia, Diana G, Calderón-Gallegos, Arturo, de la Torre, Patricia, Carrero, Julio C, Sanchez-Flores, Alejandro, Laclette, Juan P
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 10-05-2022
• Subjects: Africa; Animals; Cellular and Infection Microbiology; comparative genomics; Cysticercosis - veterinary; differential expression; Disease Models, Animal; Genomics; Humans; ORF; RNA-seq; Taenia crassiceps; Taenia solium - genetics; WFU; Africa; ORF; cysticercosis; RNA-seq; WFU; differential expression; Taenia crassiceps; animal model; comparative genomics
• ISSN: 2235-2988; 2235-2988
• DOI: 10.3389/fcimb.2022.876839

Human cysticercosis by
is the major cause of neurological illness in countries of Africa, Southeast Asia, and the Americas. Publication of four cestode genomes (
,
,
and
) in the last decade, marked the advent of novel approaches on the study of the host-parasite molecular crosstalk for cestode parasites of importance for human and animal health.
is another cestode parasite, closely related to
, which has been used in numerous studies as an animal model for human cysticercosis. Therefore, characterization of the
genome will also contribute to the understanding of the human infection. Here, we report the genome of
WFU strain, reconstructed to a noncontiguous finished resolution and performed a genomic and differential expression comparison analysis against ORF strain. Both strain genomes were sequenced using Oxford Nanopore (MinION) and Illumina technologies, achieving high quality assemblies of about 107 Mb for both strains. Dotplot comparison between WFU and ORF demonstrated that both genomes were extremely similar. Additionally, karyotyping results for both strains failed to demonstrate a difference in chromosome composition. Therefore, our results strongly support the concept that the absence of scolex in the ORF strain of
was not the result of a chromosomal loss as proposed elsewhere. Instead, it appears to be the result of subtle and extensive differences in the regulation of gene expression. Analysis of variants between the two strains identified 2,487 sites with changes distributed in 31 of 65 scaffolds. The differential expression analysis revealed that genes related to development and morphogenesis in the ORF strain might be involved in the lack of scolex formation.