The chemokine CCL5 induces CCR1-mediated hyperalgesia in mice inoculated with NCTC 2472 tumoral cells

• Published in: Neuroscience Vol. 259; pp. 113 - 125
• Main Authors: Pevida, M, Lastra, A, Meana, Á, Hidalgo, A, Baamonde, A, Menéndez, Luis
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ltd 14-02-2014; Elsevier
• Subjects: Analysis of Variance; Animals; Biological and medical sciences; bone cancer-induced pain; Bone Neoplasms - complications; CCL5; CCR1; Cell Line, Tumor; Chemokine CCL5 - antagonists & inhibitors; Chemokine CCL5 - metabolism; Disease Models, Animal; Diseases of the osteoarticular system; Dose-Response Relationship, Drug; Enzyme-Linked Immunosorbent Assay; Functional Laterality; Gene Expression Regulation, Neoplastic - physiology; hyperalgesia; Hyperalgesia - blood; Hyperalgesia - etiology; Hyperalgesia - metabolism; J113863; Male; Medical sciences; Mice; Mice, Inbred C3H; Mice, Inbred C57BL; mouse; Neoplasm Transplantation - adverse effects; Nervous system (semeiology, syndromes); Nervous system as a whole; Neurology; Pain Measurement - drug effects; Pain Threshold - drug effects; Pain Threshold - physiology; Peptide T - therapeutic use; Receptors, CCR1 - antagonists & inhibitors; Receptors, CCR1 - metabolism; Tumors of striated muscle and skeleton; Xanthenes - therapeutic use; Dulbecco’s modified Eagle’s medium; RANTES; C-C chemokine receptor type 1; phosphate-buffered saline; DMEM; regulated on activation normal T cell expressed and secreted; mouse; analysis of variance; chemokine (C-C motif) ligand 2; bone cancer-induced pain; ANOVA; macrophage inhibitory protein-1α; CCL5; ethylenediaminetetraacetic acid; MIP-1α; CCL2; fetal calf serum; CCR5; CCR1; PBS; d-Ala-peptide T-amide; dorsal root ganglia; transient receptor potential vanilloid 1; chemokine (C-C motif) ligand 5; TRPV1; EDTA; enzyme-linked immunosorbent assay; FCS; DRG; J113863; C-C chemokine receptor type 5; hyperalgesia; DAPTA; ELISA; Nervous system diseases; Diseases of the osteoarticular system; Rodentia; Hyperalgesia; Chemokine; Vertebrata; Mammalia; Pain; Mouse; Bone tumor; Animal
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2013.11.055

Highlights • CCR1 blockade inhibits the hyperalgesia evoked by NCTC 2472 but not B16-F10 cells. • The antihyperalgesic effect is mediated at tumoral level and not at the spinal cord. • Spinal and tumoral CCL3 levels are unchanged in mice inoculated with tumoral cells. • CCL5 levels are increased in tumors evoked by NCTC 2472 but not B16-F10 cells. • The administration of an anti-CCL5 antibody inhibits NCTC 2472-evoked hyperalgesia.