Involvement of insulin and growth hormone (GH) during follicular development in the bovine ovary

• Published in: Animal reproduction science Vol. 106; no. 1; pp. 143 - 152
• Main Authors: Shimizu, Takashi, Murayama, Chiaki, Sudo, Natsuko, Kawashima, Chiho, Tetsuka, Masa, Miyamoto, Akio
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-06-2008; [Amsterdam]: Elsevier Science
• Subjects: Animals; biological development; Cattle - genetics; Cattle - physiology; Cells, Cultured; Cow; cows; Drug Combinations; Estradiol - pharmacology; Female; Follicle; Follicle Stimulating Hormone - pharmacology; follicular development; follicular fluid; Follicular Fluid - chemistry; Follicular Phase - genetics; Follicular Phase - metabolism; Follicular Phase - physiology; FSH; gene expression; gene expression regulation; Gene Expression Regulation - drug effects; granulosa cells; Growth hormone; Growth Hormone - analysis; Growth Hormone - physiology; hormonal regulation; hormone receptors; hormone secretion; in vitro studies; Insulin; Insulin - analysis; Insulin - physiology; messenger RNA; Ovarian Follicle - growth & development; Ovarian Follicle - metabolism; Receptor, Insulin - genetics; Receptor, Insulin - metabolism; Receptors, Somatotropin - genetics; Receptors, Somatotropin - metabolism; RNA, Messenger - metabolism; somatotropin; temporal variation; thecal cells; Time Factors; Follicle; Cow; FSH; Growth hormone; Insulin
• ISSN: 0378-4320; 1873-2232
• DOI: 10.1016/j.anireprosci.2007.04.005

Insulin and growth hormone (GH) play critical roles in the process of follicular development and maturation. However, the involvement of insulin receptor (IR) and GH receptor (GHR) during follicular development is not well understood. The aim of this study was to investigate the expression of IR and GHR mRNAs in the granulosa cells (GCs) and theca tissues (TCs) of the follicle at different developmental stages (preovulatory dominant follicles, POFs; estrogen-active dominant follicles, EADs; estrogen-inactive dominant follicles, EIDs; and small follicles, SFs), and second, to examine the effects of follicle-stimulating hormone (FSH) and estradiol (E2) on the expression of IR and GHR genes in cultured bovine GCs. Although the concentration of insulin in follicular fluid (FF) was constant at all developmental stages, the GH concentration in FF was significantly increased in the EAD and POF compared with the EID. IR mRNA in GCs and TCs was significantly increased in the POF compared with other follicles. Regarding GHR expression, significant increases of mRNA expression were observed in GCs of EAD compared to those of SF, EID and POF. GHR mRNA in TCs was significantly decreased in the SF compared with other follicles. In cultured GCs, FSH, but not E2, stimulated the expression of IR and GHR genes. Our results suggest that the increase in the expression of GHR may be a turning point for follicles to enter the ovulatory phase during final follicular development and that the insulin system may support the maturation of preovulatory follicles.