Identification of Two Subsets of Murine DC1 Dendritic Cells That Differ by Surface Phenotype, Gene Expression, and Function

• Published in: Frontiers in immunology Vol. 12; p. 746469
• Main Authors: Hongo, David, Zheng, Pingping, Dutt, Suparna, Pawar, Rahul D, Meyer, Everett, Engleman, Edgar G, Strober, Samuel
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 26-10-2021
• Subjects: Animals; CD4 T cell; CD8 Antigens - immunology; CD8 T cell; dendritic cells; Dendritic Cells - immunology; Immunology; Lymphocyte Activation - immunology; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Phenotype; Transcriptome - immunology; tumor vaccination; type I dendritic cells; type II dendritic cell; CD8 T cell; CD4 T cell; type I dendritic cells; dendritic cells; tumor vaccination; type II dendritic cell
• ISSN: 1664-3224; 1664-3224
• DOI: 10.3389/fimmu.2021.746469

Classical dendritic cells (cDCs) in mice have been divided into 2 major subsets based on the expression of nuclear transcription factors: a CD8
Irf8
Batf3 dependent (DC1) subset, and a CD8
Irf4
(DC2) subset. We found that the CD8
DC1 subset can be further divided into CD8
DC1a and CD8
DC1b subsets by differences in surface receptors, gene expression, and function. Whereas all 3 DC subsets can act alone to induce potent Th1 cytokine responses to class I and II MHC restricted peptides derived from ovalbumin (OVA) by OT-I and OT-II transgenic T cells, only the DC1b subset could effectively present glycolipid antigens to natural killer T (NKT) cells. Vaccination with OVA protein pulsed DC1b and DC2 cells were more effective in reducing the growth of the B16-OVA melanoma as compared to pulsed DC1a cells in wild type mice. In conclusion, the Batf3-/- dependent DC1 cells can be further divided into two subsets with different immune functional profiles
and
.