Induction of mRNA for Chemokines and Chemokine Receptors by Prostaglandin F2α Is Dependent upon Stage of the Porcine Corpus Luteum and Intraluteal Progesterone

• Published in: Endocrinology (Philadelphia) Vol. 152; no. 7; pp. 2797 - 2805
• Main Authors: Luo, Wenxiang, Diaz, Francisco J, Wiltbank, Milo C
• Format: Journal Article
• Language: English
• Published: Chevy Chase, MD Endocrine Society 01-07-2011; Oxford University Press
• Subjects: 3 beta -Hydroxysteroid dehydrogenase; 3-Hydroxysteroid Dehydrogenases - antagonists & inhibitors; Androstenols - pharmacology; Animals; Biological and medical sciences; CC chemokine receptors; CCL4 protein; CCR1 protein; CCR2 protein; CCR5 protein; Chemokine receptors; Chemokines; Chemokines - genetics; Chemokines - metabolism; Corpus luteum; Corpus Luteum - metabolism; CXCR2 protein; Dinoprost - metabolism; Enzyme Inhibitors - pharmacology; Factorial design; Female; Fundamental and applied biological sciences. Psychology; Gene expression; Genes; Hydroxysteroids; Interleukin 8; Leukocyte migration; Luteinization - metabolism; Monocyte chemoattractant protein 1; mRNA; NF-kappa B - metabolism; Phosphorylation; Polymerase chain reaction; Progesterone; Progesterone - metabolism; Prostaglandin F2 alpha; Prostaglandin F2a; Prostaglandins; Protein Processing, Post-Translational; Proteins; Pseudopregnancy; Pseudopregnancy - metabolism; Random Allocation; Receptors, Chemokine - genetics; Receptors, Chemokine - metabolism; Reproduction-Development; RNA, Messenger - metabolism; Signal Transduction; Sus scrofa; Up-Regulation - drug effects; Vertebrates: endocrinology; Western blotting; Induction; Arachidonic acid derivatives; Chemokine receptor; Ovarian hormone; Progestagen; Pig; Prostaglandin F2α; Ovary; Vertebrata; Mammalia; Eicosanoid; Animal; Female genital system; Corpus luteum; Artiodactyla; Progesterone; Sex steroid hormone; Ungulata
• ISSN: 0013-7227; 1945-7170
• DOI: 10.1210/en.2010-1247

This study tested the hypotheses that prostaglandin (PG) F2α increases expression of genes related to recruitment of leukocytes in mature but not early corpus luteum (CL) and that insensitivity to PGF2α action in early CL is dependent on high intraluteal progesterone (P4) concentrations. Experiment 1 examined early (0.5 h) and late (10 h) in vivo effects of PGF2α on mature (d 17 of pseudopregnancy) and early (d 9) porcine CL. Real-time PCR was used to measure mRNA for chemokines (IL8, CXCL2, CCL2, CCL8, CCL4, CCL11) and chemokine receptors (CCR1, CCR2, CXCR2, CCR5). Western blotting was used to measure protein expression and phosphorylation of nuclear factor-κB proteins. Treatment with PGF2α for 10 h increased mRNA for almost all of these genes (all expect CXCL2 and CCL11) in d 17 CL but not d 9 CL. Treatment with PGF2α also led to greater phosphorylation of nuclear factor-κB-1A protein in d 17 than d 9 CL. Experiment 2 had a 2 × 2 factorial design with d 9 gilts treated or not treated with epostane (3β-hydroxysteroid dehydrogenase inhibitor to suppress intraluteal P4) and treated or not treated with PGF2α. Treatment with PGF2α (10 h) or epostane alone did not induce expression of any of these genes in d 9 CL. However, PGF2α + epostane increased expression of all of these genes except CCL11. In conclusion, PGF2α increases mRNA for chemokines and chemokine receptors in mature CL with similar PGF2α effects induced in early CL if intraluteal P4 is suppressed prior to PGF2α treatment.