S100A8 and S100A9 promotes invasion and migration through p38 mitogen-activated protein kinase-dependent NF-κB activation in gastric cancer cells

• Published in: Molecules and cells Vol. 35; no. 3; pp. 226 - 234
• Main Authors: Kwon, C.H., National University School of Medicine, Busan, Republic of Korea, Moon, H.J., National University School of Medicine, Busan, Republic of Korea, Park, H.J., National University School of Medicine, Busan, Republic of Korea, Choi, J.H., National University School of Medicine, Busan, Republic of Korea, Park, D.Y., National University School of Medicine, Busan, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Springer Korean Society for Molecular and Cellular Biology 01-03-2013; Korea Society for Molecular and Cellular Biology
• Subjects: Biochemistry; Biomedical and Life Sciences; Biomedicine; Biotechnology; Calgranulin A - physiology; Calgranulin B - physiology; Cell Biology; Cell Line, Tumor; Cell Movement; Enzyme Activation; gastric cancer cells,S100A8/A9,p38MAPK,NF-κB; Humans; Imidazoles - pharmacology; Life Sciences; MAP Kinase Signaling System; Matrix Metalloproteinase 12 - metabolism; Matrix Metalloproteinase 2 - metabolism; NEOPLASMAS; NEOPLASME; NEOPLASMS; NF-kappa B - metabolism; p38 Mitogen-Activated Protein Kinases - antagonists & inhibitors; p38 Mitogen-Activated Protein Kinases - metabolism; Phosphorylation; Protein Processing, Post-Translational; Pyridines - pharmacology; Research Article; Stomach Neoplasms - metabolism; Stomach Neoplasms - pathology; gastric cancer cells; MMP; NF-κB; S100A8/A9; p38MAPK
• ISSN: 1016-8478; 0219-1032
• DOI: 10.1007/s10059-013-2269-x

S100A8 and S100A9 (S100A8/A9) are low-molecular weight members of the S100 family of calcium-binding proteins. Recent studies have reported S100A8/A9 promote tumorigenesis. We have previously reported that S100A8/A9 is mostly expressed in stromal cells and inflammatory cells between gastric tumor cells. However, the role of environmental S100A8/A9 in gastric cancer has not been defined. We observed in the present study the effect of S100A8/A9 on migration and invasion of gastric cancer cells. S100A8/ A9 treatment increased migration and invasionat lower concentrations that did not affect cell proliferation and cell viability. S100A8/A9 caused activation of p38 mitogenactivated protein kinase (MAPK) and nuclear factor-κB (NF-κB). The phosphorylation of p38 MAPK was not affected by the NF-κB inhibitor Bay whereas activation of NF-κB was blocked by p38 MAPK inhibitor SB203580, indicating that S100A8/A9-induced NF-κB activation is mediated by phosphorylation of p38 MAPK. S100A8/A9-induced cell migration and invasion was inhibited by SB203580 and Bay, suggesting that activation of p38 MAPK and NF-κB is involved in the S100A8/A9 induced cell migration and invasion. S100A8/A9 caused an increase in matrix metalloproteinase 2 (MMP2) and MMP12 expression, which were inhibited by SB203580 and Bay. S100A8/A9-induced cell migration and invasion was inhibited by MMP2 siRNA and MMP12 siRNA, indicating that MMP2 and MMP12 is related to the S100A8/A9 induced cell migration and invasion. Taken together, these results suggest that S100A8/A9 promotes cell migration and invasion through p38 MAPKdependent NF-κB activation leading to an increase of MMP2 and MMP12 in gastric cancer.