Peripheral inflammatory activation after hippocampus irradiation in the rat

• Published in: International journal of radiation biology Vol. 90; no. 1; pp. 1 - 6
• Main Authors: T kés, Tünde, Varga, Gabriella, Garab, Dénes, Nagy, Zoltán, Fekete, Gábor, Tuboly, Eszter, Plangár, Imola, Mán, Imola, Szabó, Rita Emília, Szabó, Zoltán, Volford, Gábor, Ghyczy, Miklós, Kaszaki, József, Boros, Mihály, Hideghéty, Katalin
• Format: Journal Article
• Language: English
• Published: England Informa Healthcare 01-01-2014; Taylor & Francis
• Subjects: Animals; ATP; Brain irradiation; cytokines; Cytokines - blood; Glycerylphosphorylcholine - administration & dosage; Hippocampus - drug effects; Hippocampus - metabolism; Hippocampus - radiation effects; Inflammation - blood; Inflammation - prevention & control; L-alpha-glycerylphosphorylcholine; Male; Radiation Injuries - blood; Radiation Injuries - prevention & control; Radiation Tolerance - radiation effects; Radiation-Protective Agents - administration & dosage; radioprotection; Radiotherapy, Conformal - adverse effects; Rats; Rats, Sprague-Dawley; Space life sciences; TNF-alpha
• ISSN: 0955-3002; 1362-3095
• DOI: 10.3109/09553002.2013.836617

Abstract
Purpose: To detect the possible biochemical signs of inflammatory activation in the peripheral circulation in a rodent model of hippocampus irradiation, and to examine the effects of L-alpha-glycerylphosphorylcholine (GPC) in this experimental protocol.
Materials and methods: Anesthetized Sprague-Dawley rats were subjected to 40 Gy cobalt irradiation of both hemispheres of the hippocampus, with or without GPC treatment (50 mg/kg intravenously (i.v.), 5 min before the irradiation, n = 6, each). A third group (n = 6) served as saline-treated control. Blood samples were obtained 3 h after the end of irradiation in order to examine the changes in plasma histamine, tumor necrosis factor-alpha (TNF-α), interleukin 1-beta, interleukin 6 (IL-6) and interleukin 10 (IL-10); liver tissue samples were taken to determine adenosine triphosphate (ATP) concentrations.
Results: The hepatic ATP levels were significantly declined, while plasma concentrations of circulating TNF-α, IL-6, IL-10 and histamine were significantly increased after hippocampus irradiation. GPC treatment significantly reduced the irradiation-induced release of cytokines and histamine, and the liver ATP level was maintained at the control value.
Conclusions: Targeted brain irradiation produced measurable pro- and anti-inflammatory cytokine changes in the systemic circulation. GPC supplementation provides significant protection against irradiation-induced peripheral pro-inflammatory activation and ATP depletion.