Determination of the Isosbestic Point in the Absorption Spectra of DNA–Ethidium Bromide Complexes

We propose a procedure for mathematical treatment of the spectra of complexes between ligands and DNA, which allows us to determine the position of the isosbestic point with high precision. As a specific example, we consider the absorption spectra of ethidium bromide complexes titrated against a DNA...

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Bibliographic Details
Published in:	Journal of applied spectroscopy Vol. 81; no. 6; pp. 1060 - 1063
Main Authors:	Vardevanyan, P. O., Élbakyan, V. L., Shahinyan, M. A., Minasyants, M. V., Parsadanyan, M. A., Sahakyan, N. S.
Format:	Journal Article
Language:	English
Published:	Boston Springer US 01-01-2015 Springer
Subjects:	Analytical Chemistry Atomic/Molecular Structure and Spectra DNA Genetic research Physics Physics and Astronomy isosbestic point absorption spectrum DNA DNA–ligand complexes
Online Access:	Get full text
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Summary:	We propose a procedure for mathematical treatment of the spectra of complexes between ligands and DNA, which allows us to determine the position of the isosbestic point with high precision. As a specific example, we consider the absorption spectra of ethidium bromide complexes titrated against a DNA solution. As a result of using the method, besides the main type of binding we have identified the appearance of additional types of binding between the ethidium bromide and DNA.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0021-9037 1573-8647
DOI:	10.1007/s10812-015-0051-x