chloroxoquinolinic derivative 6-chloro-1,4-dihydro-4-oxo-1-(β-D-ribofuranosyl) quinoline-3-carboxylic acid inhibits HSV-1 adsorption by impairing its adsorption on HVEM

• Published in: Archives of virology Vol. 152; no. 7; pp. 1417 - 1424
• Main Authors: Souza, T. M. L, De Souza, M. C. Bastos V, Ferreira, V. F, Canuto, C. V. B. Santos, Marques, I. Pereira, Fontes, C. F. L, Frugulhetti, I. C. P. P
• Format: Journal Article
• Language: English
• Published: Wien Vienna : Springer-Verlag 01-07-2007; New York, NY Springer; Springer Nature B.V
• Subjects: Acyclovir - pharmacology; Adsorption; Animals; Antiviral Agents - chemistry; Antiviral Agents - pharmacology; Biological and medical sciences; Carboxylic acids; Cercopithecus aethiops; CHO Cells; Cricetinae; Cricetulus; Cytotoxicity; Fundamental and applied biological sciences. Psychology; Herpes simplex virus 1; Herpes viruses; Herpesvirus 1, Human - drug effects; Herpesvirus 1, Human - pathogenicity; In Vitro Techniques; Microbiology; Miscellaneous; Quinolones - chemistry; Quinolones - pharmacology; Receptors, Tumor Necrosis Factor, Member 14 - drug effects; Receptors, Tumor Necrosis Factor, Member 14 - physiology; Vero Cells; Virology; viruses; Virus; Human herpesvirus 1; Adsorption; Herpesviridae; Alphaherpesvirinae
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-007-0960-y

In this paper, we describe that the oxoquinolinic acid derivative (compound A) inhibited HSV-1 adsorption on Vero cells. This effect was achieved with an EC₅₀ value of 10 ± 2.0 μM and with low cytotoxicity, since the CC₅₀ value for compound A was >1000 μM. Moreover, we demonstrate for the first time that adsorption inhibition was due to the blockage of the interactions between HSV-1 and the cellular receptor herpes virus entry mediator (HVEM). These results show that compound A can prevent HSV-1 infection in Vero cells, encouraging further studies to determine at what level compound A inhibits HSV-1-HVEM interactions.