Effect of superoxide dismutase, catalase, and glutathione peroxidase supplementation in the extender on chilled semen of fertile and hypofertile dogs

• Published in: Journal of veterinary science (Suwŏn-si, Korea) Vol. 19; no. 5; pp. 667 - 675
• Main Authors: Prete, Chiara Del, Ciani, Francesca, Tafuri, Simona, Pasolini, Maria Pia, Valle, Giovanni Della, Palumbo, Veronica, Abbondante, Lucia, Calamo, Antonio, Barbato, Vincenza, Gualtieri, Roberto, Talevi, Riccardo, Cocchia, Natascia
• Format: Journal Article
• Language: English
• Published: Korea (South) 대한수의학회 01-09-2018; The Korean Society of Veterinary Science
• Subjects: Original; 수의학; extender; canine; chilled semen; semen preservation; antioxidants
• ISSN: 1229-845X; 1976-555X
• DOI: 10.4142/jvs.2018.19.5.667

This study investigated the correlation between oxidative stress status and main canine sperm parameters and the effect of addition of Superoxide Dismutase (SOD), Catalase (CAT) and Glutathione Peroxidase (GPx) combination in egg yolk tris-citrate glucose (EYT-G) extender on semen during 10 days of storage at 4° C. Ten Boxer dogs were included in this study and divided in two groups, fertile (F) and hypofertile (H), depending on pregnancy and live birth rates in the previous year. The semen evaluation was performed at the day of collection (D0) and after 5 (D5) and 10 (D10) days of cooled storage at 5° C. Sperm motility, kinetic parameters and DNA integrity were assessed. A correlation between oxidative status and the main semen parameters in both F and H groups was found. Total and progressive motility were significantly higher in treated (SOD, CAT and GPx addition) versus control groups at D10 in both F and H groups, and at D5 in H group. DNA integrity was significantly higher in both treated groups (H and F) at D5 and D10. In conclusion, the addition of SOD, CAT and GPx in the extender has allowed the preservation of semen quality up to 10 days of storage at 5° C both in fertile and hypofertile dogs.