Decrease in CA3 inhibitory network activity during Theiler’s virus encephalitis

• Published in: Neuroscience letters Vol. 609; pp. 210 - 215
• Main Authors: Smeal, R.M., Fujinami, R., White, H.S., Wilcox, K.S.
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 16-11-2015
• Subjects: Animals; CA3; CA3 Region, Hippocampal - physiopathology; Cardiovirus Infections - physiopathology; Cardiovirus Infections - virology; Epilepsy; Epilepsy - physiopathology; Epilepsy - virology; gamma-Aminobutyric Acid - physiology; Inhibitory Postsynaptic Potentials; IPSCs; Male; Mesial temporal sclerosis; Mice, Inbred C57BL; Mouse model; Pyramidal Cells - physiology; Theilovirus; Time Factors; Viral encephalitis; Mouse model; Mesial temporal sclerosis; CA3; IPSCs; Epilepsy; Viral encephalitis
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/j.neulet.2015.10.032

•A mouse model of virus-induced epilepsy is used to explore inhibitory circuit changes.•IPSC amplitudes are reduced during acute infection but recover at the chronic condition.•mIPSC amplitudes are similarly reduced during the acute infection but not chronically.•mIPSC kinetics demonstrate subtle changes following TMEV infection.
Viral infections of the central nervous system are often associated with seizures, and while patients usually recover from the infection and the seizures cease, there is an increased lifetime incidence of epilepsy. These viral infections can result in mesial temporal sclerosis, and, subsequently, a type of epilepsy that is difficult to treat. In previous work, we have shown that Theiler’s murine encephalomyelitis virus (TMEV) infections in C57B/6 mice, an animal model of virus-induced epilepsy, results in changes in excitatory currents of CA3 neurons both during the acute infection and two months later, at a time when seizure thresholds are reduced and when spontaneous seizures can occur. The changes in the excitatory system differ at these two time points, suggesting different mechanisms for seizure generation. In the present paper, we examine GABAergic mediated inhibition in CA3 pyramidal cells at these two time points following TMEV infection. We found that amplitudes of sIPSCs and mIPSCs were reduced during the acute infection, but recovered at the two-month time point. These observations are consistent with previous measurements of excitatory currents suggesting different mechanisms of seizure generation during the acute infection and during chronic epilepsy.