Activities of MSCs Derived from Transgenic Mice Seeded on ADM Scaffolds in Wound Healing and Assessment by Advanced Optical Techniques

• Published in: Cellular physiology and biochemistry Vol. 42; no. 2; pp. 623 - 639
• Main Authors: Wang, Ning, Liu, Hanping, Li, Xuefeng, Zhang, Qilei, Chen, Maosheng, Jin, Ying, Deng, Xiaoyuan
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland S. Karger AG 01-01-2017; Cell Physiol Biochem Press GmbH & Co KG
• Subjects: Actins - biosynthesis; ADM scaffolds; Alcohol; Animals; Bone marrow; Bone Marrow Cells; Cell adhesion & migration; Cell Proliferation - genetics; Collagen; Cutaneous wound healing; Flow cytometry; GFP-labeling; Humans; Labeling; Laboratory animals; Mesenchymal Stem Cell Transplantation; Mesenchymal Stem Cells - cytology; Methods; Mice; Mice, Transgenic; MSCs; Original Paper; Regeneration - genetics; Rodents; SHG-TPEF imaging; Skin & tissue grafts; Stem cells; Tissue Engineering; Tissue Scaffolds; Transgenic animals; Transplants & implants; Wound healing; Wound Healing - genetics; China; MSCs; GFP-labeling; SHG-TPEF imaging; Cutaneous wound healing; ADM scaffolds
• ISSN: 1015-8987; 1421-9778
• DOI: 10.1159/000477872

Background/Aims: Bone marrow Mesenchymal stem cells (MSCs) are promising for promoting cutaneous wound healing through reinforcing cellular processes. We evaluated the effect of GFP-tagged MSCs transplantation on skin regeneration in excisional wounds in mice. Methods: MSCs from GFP-labeled transgenic mice were co-cultured with acellular dermal matrix (ADM) scaffolds, and MSC-ADM scaffolds were transplanted into surgical skin wounds of BALB/c mice. After implantation, the survival and behavior of MSCs were examined by second harmonic generation and two-photon excitation fluorescence imaging, western blotting and DNA amplification and sequencing. Results: GFP-tagged MSCs were retained inside the regenerating skin until day 14 post-transplantation. Alpha-smooth muscle actin (α-SMA) and vimentin (VIM) were detected at 3, 5, 7, and 14 days post-transplantation by immunofluorescence double labeling. Although the GFP + /α-SMA + - and GFP + /VIM + -cell numbers decreased gradually with healing time, α-SMA + - and VIM + -cell numbers significantly increased, most of them were endogenous functional cells which were related to angiogenesis and collagen fiber structural remodeling. Conclusion: Therefore, in the initial stage of wound healing, transplanted MSCs differentiated into functional cells and played paracrine roles to recruit more endogenous cells for tissue remodeling. With the disappearance of exogenous cells, endogenous cells were responsible for the latter stage of cutaneous wound healing.