Validation of the Short Amplicon Multiplex Q8 Including the German DNA Database Systems

• Published in: Journal of forensic sciences Vol. 54; no. 4; pp. 862 - 865
• Main Authors: Müller, Kathrin, Braunschweiger, Günter, Klein, Rachel, Miltner, Erich, Wiegand, Peter
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01-07-2009; Wiley Subscription Services, Inc
• Subjects: amelogenin; Amelogenin - genetics; D18S51; D21S11; D3S1358; D8S1179; Databases, Nucleic Acid; Deoxyribonucleic acid; DNA; DNA degradation; DNA Degradation, Necrotic; DNA Primers; DNA typing; FGA; Forensic Genetics - methods; forensic science; Forensic sciences; Genetics, Population; German DNA database systems; Germany; Humans; low copy number; Polymerase Chain Reaction - methods; SE33; short amplicon multiplex; Tandem Repeat Sequences; TH01; VWA; Germany
• ISSN: 0022-1198; 1556-4029
• DOI: 10.1111/j.1556-4029.2009.01066.x

:  We have developed a concept to enable the analyzing of degraded stains with limited DNA template quantity. Therefore we have constructed a short tandem repeat (STR) multiplex including the German DNA database systems (Q8). The amplicon lengths are smaller than 280 bp. For the validation of Q8 over 50 degraded samples were investigated. Amplifications were performed with “low copy number” PCR, the number of PCR cycles was increased to 33 and the reaction volume was decreased to 12.5 μL. Compared with the MPX2 and Nonaplex kit, the average success rate was increased using the Q8 kit by approximately 20% and 30%, respectively. The efficiency of a sensitive STR multiplex with reduced amplicon lengths was confirmed in comparing the success rates of Q8 for typing degraded samples and samples with limited amount of DNA template while partial profiles were observed with the majority of the samples using commercially available kits.