Evaluation of microagglutination test for differentiation between Serpulina (Treponema) hyodysenteriae and S. innocens and serotyping of S. hyodysenteriae

• Published in: Journal of Clinical Microbiology Vol. 32; no. 8; pp. 1976 - 1979
• Main Authors: Diarra, A.T. (University of Montreal, Quebec, Canada.), Mittal, K.R, Achacha, M
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01-08-1994
• Subjects: Agglutination Tests - methods; Antigens, Bacterial; Bacteriological methods and techniques used in bacteriology; Bacteriology; Biological and medical sciences; Brachyspira - classification; Brachyspira - immunology; Brachyspira hyodysenteriae - classification; Brachyspira hyodysenteriae - immunology; DIAGNOSTIC; DIAGNOSTICO; Fundamental and applied biological sciences. Psychology; Immunodiffusion; Microbiology; REACCIONES DE AGLUTINACION; REACTION D'AGGLUTINATION; Reference Standards; SEROTIPOS; SEROTYPE; Serotyping - methods; Serpulina hyodysenteriae; TREPONEMA; Spirochaetales; Bacteria; Agglutination test; Identification; Species specificity; Method; Serotype; Serpulina hyodysenteriae; Serotyping
• ISSN: 0095-1137; 1098-660X
• DOI: 10.1128/jcm.32.8.1976-1979.1994

Swine dysentery is a mucohemorrhagic diarrheal disease caused by the anaerobic spirochete Serpulina hyodysenteriae. At present, the serotyping is done by immunodiffusion testing with lipopolysaccharide (LPS) extract as antigen and rabbit hyperimmune sera produced against different serotypes of S. hyodysenteriae. Since the preparation of LPS is time-consuming and requires a large quantity of bacteria, it is desirable to use a serotyping method which does not require the extraction of LPS. In the present investigation, microagglutination was evaluated by using both formalinized whole- and boiled-cell suspensions as antigens and rabbit hyperimmune sera produced against formalinized whole-cell suspensions of reference strains of S. hyodysenteriae and S. innocens B256. Use of boiled cell suspension as antigen permitted the differentiation between isolates of S. hyodysenteriae and S. innocens as well as serotyping of S. hyodysenteriae strains accurately. A total of 18 isolates were identified as S. hyodysenteriae, and 3 isolates were identified as S. innocens. The microagglutination test was found specific, sensitive, and easy to perform; thus, it was judged suitable for routine identification and serotyping of S. hyodysenteriae isolates