Surface modification by argon plasma treatment improves antioxidant defense ability of CHO-k1 cells on titanium surfaces

• Published in: Toxicology in vitro Vol. 28; no. 3; pp. 381 - 387
• Main Authors: Queiroz, Jana Dara Freires de, Leal, Angélica Maria de Sousa, Terada, Maysa, Agnez-Lima, Lucymara Fassarela, Costa, Isolda, Pinto, Nadja Cristhina de Souza, Batistuzzo de Medeiros, Silvia Regina
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-04-2014
• Subjects: Animals; Antioxidants - metabolism; Argon - chemistry; Biocompatible Materials - chemistry; Biomaterial; Cell Survival; CHO Cells; Cricetinae; Cricetulus; DNA Damage; Oxidative Stress; Plasma treatment; Prostheses and Implants; Titanium; Titanium - chemistry; PBS; Oxidative stress; DNPH; Plasma treatment; DMSO; CHO-k1; PTTS; SOD; EDTA; MTT; BSA; DMEM; NC; UTS; FBS; CAT; ROS; Titanium; PI; Biomaterial; Fpg
• ISSN: 0887-2333; 1879-3177
• DOI: 10.1016/j.tiv.2013.11.012

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•Plasma treated titanium is better to cell viability than untreated titanium.•Cellular antioxidant response was more efficient on plasma treated titanium.•Plasma treatment could improve the titanium biocompatibility.
Titanium is one of the most used materials in implants and changes in its surface can modify the cellular functional response to better implant fixation. An argon plasma treatment generates a surface with improved mechanical proprieties without modifying its chemical composition. Oxidative stress induced by biomaterials is considered one of the major causes of implant failure and studies in this field are fundamental to evaluate the biocompatibility of a new material. Therefore, in this work, induction of oxidative stress by titanium surfaces subjected to plasma treatment (PTTS) was evaluated. The viability of CHO-k1 cells was higher on PTTS discs. Cells grown on titanium surfaces are subjected to intracellular oxidative stress. Titanium discs subjected to the plasma treatment induced less oxidative stress than the untreated ones, which resulted in improved cellular survival. These were associated with improved cellular antioxidant response in Plasma Treated Titanium Surface (PTTS). Furthermore, a decrease in protein and DNA oxidative damage was observed on cells grown on the roughed surface when compared to the smooth one. In conclusion, our data suggest that the treatment of titanium with argon plasma may improve its biocompatible, thus improving its performance as implants or as a scaffold in tissue engineering.