Molecular Characterization and Chemotactic Function of CXCL8 in Northeast Chinese Lamprey ( Lethenteron morii )

Chemokine-induced chemotaxis of leukocytes is an important part of the innate immunity and has been shown to mediate inflammation in all groups of jawed vertebrates. For jawless vertebrates, hagfish leukocytes are known to show chemotaxis toward mammalian complement anaphylotoxin and Gram-negative b...

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Published in:Frontiers in immunology Vol. 11; p. 1738
Main Authors: Zhu, Xinyun, Zhang, Zhe, Ren, Jianfeng, Jia, Liang, Ding, Shaoqing, Pu, Jiafei, Ma, Wenyuan, Tao, Yan, Zu, Yao, Li, Weiming, Zhang, Qinghua
Format: Journal Article
Language:English
Published: Switzerland Frontiers Media S.A 18-08-2020
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Summary:Chemokine-induced chemotaxis of leukocytes is an important part of the innate immunity and has been shown to mediate inflammation in all groups of jawed vertebrates. For jawless vertebrates, hagfish leukocytes are known to show chemotaxis toward mammalian complement anaphylotoxin and Gram-negative bacteria lipopolysaccharide. However, whether chemokines mediate chemotaxis of leukocytes in jawless vertebrates has not been conclusively examined. Here, we show C-X-C motif chemokine ligand 8 (CXCL8, also named interleukin 8) of the Northeast Chinese lamprey ( ) (designated as LmCXCL8) induces chemotaxis in its leukocytes. We identified LmCXCL8 and found it possesses the characteristic N-terminal cysteine residues and GGR (Gly-Gly-Arg) motif. The gene was found to be expressed in all examined tissues, and its expression was inducible in the lamprey challenged by an infectious bacterium, . A recombinant LmCXCL8 protein elicited concentration-dependent chemotaxis in peripheral blood leukocytes isolated from the Northeast Chinese lamprey. Based on these results, we conclude that LmCXCL8 is a constitutive and inducible acute-phase cytokine that mediates immune defense and trace the chemotactic function of chemokine to basal vertebrates.
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This article was submitted to Molecular Innate Immunity, a section of the journal Frontiers in Immunology
These authors have contributed equally to this work
Reviewed by: Daniel Medeiros, University of Colorado Boulder, United States; Sabyasachi Das, Emory University, United States
Edited by: Alexandre Corthay, Oslo University Hospital, Norway
ISSN:1664-3224
1664-3224
DOI:10.3389/fimmu.2020.01738