Efficiency of Different Disinfectants on Bacillus cereus Sensu Stricto Biofilms on Stainless-Steel Surfaces in Contact With Milk

• Published in: Frontiers in microbiology Vol. 9; p. 2934
• Main Authors: Silva, Higor Oliveira, Lima, Joyce Aparecida Santos, Aguilar, Carlos Eduardo Gamero, Rossi, Gabriel Augusto Marques, Mathias, Luis Antonio, Vidal, Ana Maria Centola
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 28-11-2018
• Subjects: biofilm formation; food safety; Microbiology; peracetic acid; recontamination; sodium hypochlorite; food safety; biofilm formation; sodium hypochlorite; recontamination; peracetic acid
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2018.02934

The species of the
group have the ability to adhere to and form biofilms on solid surfaces, including stainless steel, a material widely used in food industries. Biofilms allow for recontamination during food processing, and the "clean-in-place" (CIP) system is largely used by industries to control them. This study thus proposes to evaluate the efficacy of peracetic acid and sodium hypochlorite against biofilms induced on stainless-steel surfaces. The SAMN07414939 isolate (BioProject PRJNA390851), a recognized biofilm producer, was selected for biofilm induction on AISI 304 stainless steel. Biofilm induction was performed and classified into three categories: TCP (Tindalized, Contaminated, and Pasteurized milk), TCS (Tindalized milk Contaminated with Spores), and TCV (Tindalized milk Contaminated with Vegetative cells). Subsequently, the coupons were sanitized simulating a CIP procedure, on a pilot scale, using alkaline and acid solutions followed by disinfectants (peracetic acid and sodium hypochlorite). Microorganism adhesion on the surfaces reached 6.3 × 10
to 3.1 × 10
CFU/cm
. Results did not show significant differences (
> 0.05) for surface adhesion between the three tested categories (TCP, TCS, and TCV) or (
> 0.05) between the two disinfectants (peracetic acid and sodium hypochlorite). Microbial populations adhered to the stainless-steel coupons are equally reduced after treatment with peracetic acid and sodium hypochlorite, with no differences in the control of
biofilms on AISI 304 stainless-steel surfaces.